LAMP技术检测结核分枝杆菌的临床时效性分析  

Clinical efficiency analysis of Mycobacterium tuberculosis detected by LAMP technique

作　　者：陈伊[1] 黄文焰[1] 邓君丽[1] CHEN Yi;HUANG Wenyan;DENG Junli(Department of Clinical Laboratory,Baoan District Chronic Disease Control Institute,Shenzhen,Guangdong 518102,China)

机构地区：[1]深圳市宝安区慢性病防治院检验科,广东深圳518102

出　　处：《国际检验医学杂志》2018年第13期1622-1624,1628,共4页International Journal of Laboratory Medicine

基　　金：深圳市宝安区医疗卫生科研立项项目(2016CX223)

摘　　要：目的探讨环介导等温扩增法(LAMP)检测结核分枝杆菌在基层诊疗机构中的临床应用价值。方法采用LAMP法及抗酸染色涂片法对140例临床诊断为肺结核的患者的痰标本进行检测。统计分析采用McNemar检验和Kappa检验。结果 140例痰标本中,抗酸染色涂片法及LAMP法的阳性检出率分别为29.3%(41/140)和45.0%(63/140)。与抗酸染色涂片法比较,LAMP法的灵敏度为97.6%(40/41)、特异度为76.8%(76/99),总体一致率82.9%(116/140)。两种方法一致性相比,差异有统计学意义(Kappa=0.642,P<0.05),两者一致性良好。在检测时间上,与涂片法相比,大批量标本检测中LAMP法所用时间较短。结论痰标本检测中,LAMP法阳性检出率优于抗酸染色涂片法,批量检测性好,具有较好的临床应用前景。Objective To explore the clinical value of a loop-mediated isothermal amplification（LAMP）method for detection of Mycobacterium tuberculosis in the application of primary medical institutions.Methods The sputum samples from 140 patients with clinically diagnosed pulmonary tuberculosis detected by LAMP and anti-acid staining method.McNemar test and Kappa test were used for statistical analysis.Results In 140 cases of sputum,the positive detection rate of anti-acid staining and LAMP method was29.3%（41/140）and 45.0%（63/140）.Compared with the smear method,the sensitivity of LAMP method was97.6%（40/41）,the specificity was 76.8%（76/99）.The overall consensus rate was 82.9%（116/140）.Compared with the two methods,the consistency between the two is moderate and statistically significant（Kappa=0.642,P〈0.05）.Compared with the smear method,the LAMP method used in large-scale specimen examination takes shorter time.Conclusion In sputum sample detection,positive detection rate of LAMP method is better than antacid staining smear method.It has good batch detection ability and good clinical application prospect.

关 键 词：结核分枝杆菌 环介导等温扩增 结核 诊断 

分 类 号：R446.5[医药卫生—诊断学]