高相对分子质量脂联素对骨关节炎软骨细胞凋亡的影响  

Effect of high-molecular-weight adiponectin on apoptosis of chondrocytes in osteoarthritis

作　　者：郝东升[1] 贾俊青[1] 李慧[1] 岳忠娟 毕树雄[1] Hao Dongsheng;Jia Junqing;Li Hui;Yue Zhongjuan;Bi Shuxiong(Department of Orthopedics,Shanxi Dayi Hospital,Shanxi Academy of Medical Sciences,Taiyuan 030032,China)

机构地区：[1]山西医学科学院山西大医院骨科,太原030032

出　　处：《中国药物与临床》2018年第6期874-876,共3页Chinese Remedies & Clinics

基　　金：山西省自然科学基金(2013011047-2)

摘　　要：目的研究高相对分子质量脂联素对骨关节炎软骨细胞凋亡的影响。方法体外培养骨关节炎软骨细胞,TNF-α及IL-1β联合诱导凋亡,分别加入0.2μg/ml、1μg/ml及5μg/ml浓度的高相对分子质量脂联素干预,Annexin V-FITC/PI双染法观察高分子量脂联素对软骨细胞凋亡的影响。结果 TNF-α与IL-1β联合诱导软骨细胞12 h后,细胞凋亡百分比达10.9%,活细胞百分比为84.4%;加入高相对分子质量脂联素干预,0.2μg/ml组的凋亡细胞百分比无明显变化;1μg/ml组凋亡细胞百分比下降为3.9%,活细胞百分比上升为88.5%;5μg/ml组凋亡细胞百分比上升为7.3%,活细胞百分比下降为83.2%。结论高相对分子质量脂联素在OA中起到双向调节作用,抗凋亡抑或促凋亡作用与剂量相关。Objective To study the effect of high molecular weight adiponectin on apoptosis of chondrocyte in osteoarthritis. Methods Osteoarthritis-derived ehondroeytes were cultured in vitro,subject to induced apoptosis by TNF-α plus IL-1β. High-molecular weight adiponeetin at concentrations of 0.2 μg/ml, 1 μg/ml,and 5 μg/ml was added as intervention, respectively. Annexin V-FITC/PI double staining was used to examine the effect of high molecular weight adiponeetin on ehondroeyte apoptosis. Results After 12 h of indueetion by TNF-α plus IL-1β, the percentage of ehondroeytes apoptosis was 10.9%, and that of viable ceils was 84.4%. The percentage of apoptotie cells in the 0.2 μg/ml group did not change significantly after intervention with high-molecular-weight adiponeetin. In the 1 μg/ml group, the percentage of apoptotie cells decreased by 3.9%, and that of viable cells increased by 88.5%. In the 5 μg/ml group,the percentage of apoptotie cells increased by 7.3% ,and that of viable cells decreased to 83.2%. Conclusion High molecular weight adiponeetin plays a bidirectional role in osteoarthritis. It may inhibit or promote cell apoptosis, depending on the dose.

关 键 词：骨关节炎 脂联素 细胞凋亡 

分 类 号：R684.3[医药卫生—骨科学]