桔小实蝇中国与马来西亚种群间杂交：融合性、生物学及检疫挑战(英文)  

Hybridization of Chinese and Malaysian populations of Bactrocera dorsalis(Hendel): Compatibility,biology,and future challenges

作　　者：张灿 Muhammad Z Ahmed[3] 马骏 武目涛[1] 何日荣[1] 梁帆[1] 赵菊鹏[1] 林莉[1] 胡学难[1] Catharine Mannion[4] Muhammad Ashfaq ZHANG Can;Muhammad Z Ahmed;MA Jun;WU Mu-Tao;HE Ri-Rong;LIANG Fan;ZHAO Ju-Peng;LIN Li;HU Xue-Nan;Catharine Mannion;Muhammad Ashfaq(Guangdong Inspection and Quarantine Technology Center,Guangzliou 510623,China;Department of Entomology,South China Agricultural University,Guangzhou 510642,China;Florida Department of Agriculture and Consumer Services,Division of Plant Industry,Gainesville,Florida,USA;Tropical Researchand Education Center,University of Florida,Homestead,USA;Biodiversity Institute of Ontario,University of Guelph,Guelph,Ontario,Canada)

机构地区：[1]广东检验检疫技术中心,广州510623 [2]华南农业大学昆虫学系/生物防治教育部工程研究中心,广州510642 [3]佛罗里达农业和消费者服务部,美国佛罗里达盖恩斯维尔 [4]佛罗里达大学热带研究和教育中心,美国佛罗里达霍姆斯特德 [5]圭尔夫大学,安大略生物多样性研究所,加拿大安大略圭尔夫

出　　处：《环境昆虫学报》2018年第3期553-570,共18页Journal of Environmental Entomology

基　　金：国家重点研发计划项目(2017YFC1200600); 国家自然基金项目(31171909)

摘　　要：桔小实蝇是重要的检疫性害虫,可对水果产业造成巨大经济损失。本研究首先采用微卫星标记比较桔小实蝇不同地理种群间的遗传差异性,然后通过室内种间杂交试验,研究了两个相异地理种群(此前称谓的来自中国的桔小实蝇和来自马来西亚的木瓜实蝇)之间杂交后代在繁殖力、孵化率、发育历期、产卵管长度以及抗逆性等方面的适应性,结果表明桔小实蝇中国和马来西亚种群之间的遗传关系极为相近,不存在生殖隔离,但杂交后代在繁殖力、孵化率、幼虫发育情况、高蛹期抗逆性等方面均显著高于其亲本种群,产卵管长度则介于亲本的马来西亚种群和中国种群之间。上述杂交种的适应性特征分析表明,杂交后代可能具有比亲本更强的传播潜力,因此,本研究结果对于桔小实蝇相异地理种群的检疫防控提出了新的挑战。Bactrocera dorsalis s. s.（Hendel）（Diptera： Tephritidae） is a pestiferous and invasive tephritid fruit fly and has caused heavy losses of agricultural production. In this study,we studied the population genetics of B. dorsalis s. s. from different geographic regions. In addition,we studied the hybridization of two geographically isolated populations,one in China（B. dorsalis,called here Chinesedorsalis） and other in Malaysia（previously called B. papayae,called here Malaysian dorsalis） to see if hybrids exhibit invasive characteristics. We first studied the mating compatibilities and mating rhythms of these two populations and then performed the comparisons of their fecundity, hatchability, larval development,aculeus length,and the resistance to water with their hybrids. Microsatellite data analyses indicated low genetic differences between the B. dorsalis populations. Chinese and Malaysian dorsalis populations are fully compatible for mating, without evidence of any reproductive isolation. The fecundity,hatchability and larval development of hybrids are significantly higher than their parent population. The length of the aculeus for hybrid progenies ranged between Malaysian and Chinese dorsalis. When immersing in the water,hybrid pupal of 48 hours old showed higher survival rate as compared to their parents. Characteristics of the hybrids indicate they may have potential to become more pestiferous than their parents. Therefore,stricter regulations on the quarantine measurements used in the global fruit trade will be necessary.

关 键 词：桔小实蝇 木瓜实蝇 种群遗传学 杂交 

分 类 号：Q963[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]