苹果蠹蛾8个烟碱型乙酰胆碱受体基因的克隆与生物信息学分析  

Cloning and bioinformatics analysis of eight nicotinic acetylcholine receptor genes in Cydia pomonella

作　　者：郭金梦 罗嘉鹏 冯丽凯[3] 李飞[2] 林克剑[1] 王桂荣[1] GUO Jin-Meng;LUO Jia-Peng;FENG Li-Kai;LI Fei;LINKe-Jian;WANG Gui-Rong(State Key Laboratory of Plant Disease and Insect Pests,Institute of Plant Protection,CAgricultural Sciences,Beijing 100094,C hina;Institute of Insect Science,Zhejiang University,Hangzhou 310058,China;Institute of Plant Protection,Xinjiang Academy of Agricultural andReclamtaion Sciences,Shihezi 832000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]浙江大学昆虫科学研究所,杭州310058 [3]新疆农垦科学院植物保护研究所,新疆石河子832000

出　　处：《环境昆虫学报》2018年第3期624-632,共9页Journal of Environmental Entomology

基　　金：国家自然科学基金面上项目(31672033)

摘　　要：烟碱型乙酰胆碱受体(nicotinic acetylcholine receptors,n AChRs)能够快速介导胆碱能突触传递,并在许多认知功能障碍性疾病的过程中发挥作用。昆虫的烟碱型乙酰胆碱受体是新烟碱类等杀虫剂的重要作用靶标,而且靶标抗性是昆虫产生抗药性的一个重要机制。本文利用生物信息学结合现代分子生物学技术,以新疆石河子地区的苹果蠹蛾种群为材料,克隆获得了8个烟碱型乙酰胆碱受体n AChR亚基基因的c DNA全长序列,并对这8个亚基基因的序列进行了生物信息学分析。研究结果有助于进一步深入解析苹果蠹蛾对新烟碱类杀虫剂的靶标抗性机制,进而为开发新型靶标药剂提供科学依据和理论基础。The nicotinic acetylcholine receptor（nicotinic acetylcholine receptors,n AChRs） can quickly mediate the transmission of cholinergic synapses and play a role in the process of many cognitive dysfunction diseases. Insect n AChRs are the molecular targets of neonicotinoids and other insecticides,and the target resistance is an important mechanism for insect resistance. In this paper, using bioinformatics and modern molecular biology technology,the full-length c DNA sequences of eight n AChR subunit genes were cloned from a codling moth strain of Shihezi in Xinjiang Uygur Autonomous Region,and the sequence of the eight subunits were analyzed by bioinformatics. The results provided a theoretical basis for further analysis of the resistance mechanism of new nicotinic insecticides and development of new insecticides.

关 键 词：苹果蠹蛾 烟碱型乙酰胆碱受体 基因克隆 生物信息学分析 

分 类 号：Q963[生物学—昆虫学] S433.4[农业科学—农业昆虫与害虫防治]