基于8-羟基久洛尼定的荧光探针用于谷胱甘肽的特异性检测研究  

Specific detection of glutathione with a fluorescent probe based on 8-hydroxyjulolidine skeleton

作　　者：王超 闫钰佳 张诣为 武雨珽 代方方 WANG Chao;YAN Yu jia;ZHANG Yi wei;WU Yu ting;DAI Fang fang(College of Chemistry and Chemical Engineering,Shaanxi University of Science ＆ Technology,Xi＇an 710021,China;School of Chemical Engineering,Northwest University,Xi＇an 710069,China)

机构地区：[1]陕西科技大学化学与化工学院,陕西西安710021 [2]西北大学化工学院,陕西西安710069

出　　处：《陕西科技大学学报》2018年第4期71-75,81,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(21708023);陕西科技大学博士科研启动基金项目(2017GBJ-04)

摘　　要：还原型谷胱甘肽(GSH)是一种由三个氨基酸组成的小分子肽,是细胞与组织内重要的活性分子,与多种生理和病理功能相关.以8-羟基久洛尼定为骨架,设计、合成了一例基于香豆素荧光团的GSH荧光探针,利用8-羟基久洛尼定的刚性平面结构增强荧光母体的平面性,以实现荧光波长的红移.光谱分析表明该荧光探针对GSH具有较高的特异性,对GSH呈现典型的比率型响应特征,在516nm处的荧光强度逐渐减弱,561nm处荧光强度逐渐增强.同时,荧光强度与GSH浓度呈现较好的线性关系,表明该探针可以实现GSH的定量检测.Reduced glutathione（GSH）is a small molecule peptide consisting of three amino acids,which is an important active molecule in cells and tissues,associated with a variety of physiological and pathological functions.In this paper,a GSH fluorescent probe based on coumarin fluorophore was designed and synthesized by using 8-hydroxyjulolidine skeleton.The planarity of the fluorophore was enhanced by the rigid planar structure of 8-hydroxyjulolidine,which achieves a red shift of the fluorescence wavelength.The spectral analysis indicates that the fluorescence probe has a high specific response to GSH.The gradual decrease of the fluorescence at 516 nm and increase at 561 nm reveal a typical ratiometric response to GSH.Meanwhile,the good linear relation between the fluorescent intensity and concentration of GSH indicates that the probe can be used for quantitative detection of GSH.

关 键 词：还原型谷胱甘肽 荧光探针 8-羟基久洛尼定 香豆素 比率 

分 类 号：O657.3[理学—分析化学] TQ617.3[理学—化学]