红花对兔心肌缺血-再灌注损伤的保护作用  

Protective effects of Carthamus tinctorius on myocardial ischemia-reperfusion injury in rabbits

作　　者：张磊[1] 刘志强 徐祥文 ZHANG Lei;LIU Zhiqiang;XU Xiangwen(Rizhao People＇s Hospital,Rizhao,276000,China;College of Pharmacy,Jining Medical University 3,120 Emergeuey Rescue Command Center of Rizhao City,Rizhao 276826,China)

机构地区：[1]日照市人民医院,日照276000 [2]济宁医学院药学院 [3]日照市120紧急救援指挥中心,日照276826

出　　处：《济宁医学院学报》2018年第4期238-241,共4页Journal of Jining Medical University

基　　金：2012年济宁市科技局项目

摘　　要：目的探讨红花对兔心肌缺血-再灌注损伤的保护作用。方法家兔随机分成3组:假手术组,缺血-再灌注损伤组,红花组,每组10只。ECG-1150心电图机观察心电图,检测血清乳酸脱氢酶(LDH)、肌酸肌酶(CK)变化,免疫组化检测BCL-2蛋白表达,HE染色观察心肌细胞损伤情况并观察心肌梗死范围。结果与假手术组相比,缺血-再灌注损伤组CK和LDH水平显著增加,差异有统计学意义(P<0.05);与假手术组相比,缺血-再灌注损伤组BCL-2蛋白阳性细胞数显著增多,差异有统计学意义(P<0.05),缺血-再灌注损伤组心肌梗死范围增大,心肌细胞损伤显著;与缺血-再灌注损伤组CK和LDH水平相比,红花组CK和LDH水平显著降低差异有统计学意义(P<0.05);与缺血-再灌注损伤组BCL-2蛋白阳性细胞数相比,红花组BCL-2蛋白阳性细胞数显著减少,差异有统计学意义(P<0.05),红花组心肌梗死范围减小,心肌细胞损伤减轻。结论红花对兔心肌缺血-再灌注损伤有保护作用。Objective To explore the protective effect of Carthamus tinctorius on myocardial ischemia-reperfusion injury in rabbits.Methods Rabbits were randomly divided into 3 groups （n=10）：Sham operation group,Ischemia-reperfusion injury group,Carthamus tinctorius group.Ecg-1150 electrocardiogram machine was used to observe the electrocardiogram and lactate dehydrogenase（LDH）,Creatine Kinase（CK） changes,immunohistochemical expression of BCL-2 protein,pathological sections HE staining were used to observe myocardial cells injuries and myocardial infarct size measurements.Results Compared with in the sham operation group,the levels of CK and LDH in the ischemia-reperfusion injury group increased significantly （P〈0.05）.Compared with the sham operation group,ischemia-reperfusion injury group the BCL-2 protein positive cells count significantly increased （P〈0.05）.The myocardial infarction in ischemia-reperfusion injury group had increased scope,and myocardial cells were injuried significantly.Compared with the ischemia-reperfusion injury group,the significant decreases occurred in CK and levels in the Carthamus tinctorius group were statistically significant （P〈0.05）.In the ischemia-reperfusion injury group the BCL-2 protein positive cell number was reduced compared with carthamus tinctorius group.The difference was statistically significant （P〈0.05）.Carthamus tinctorius group of myocardial infarction range decreased and the damage of myocardial cells were alleviated.Conclusion Carthamus tinctorius has protective effect on myocardial ischemia reperfusion injury in rabbits.

关 键 词：红花 缺血-再灌注损伤 兔 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学]