基于“四时五藏阴阳”理论整体论思想的研究不同含氧量对大鼠肺、肠组织VIP、CCK及P物质表达影响的实验观察  

Experimental Observation on Effects of Different Oxygen Contents on the Expression of VIP,CCK and P Substances in the Lung and Intestine of Rats Based on“Yin-yang of the Four Seasons and the Five Viscera”

作　　者：李立华 邱新萍 郭霞珍[2] Li Lihua;Qiu Xinping;Guo Xiazhen(Shunyi Hospital of Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医医院顺义医院,北京101300 [2]北京中医药大学,北京100029

出　　处：《世界中医药》2018年第5期1095-1099,共5页World Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2009CB522706);国家自然科学基金面上基金项目(81573840)

摘　　要：目的:比较大鼠在接受不同的氧含量的刺激下,肺与回肠、结肠、空肠组织上的血管活性肠肽、P物质、胆囊收缩素的不同,探讨中医藏象的科学内涵,以及"四时五藏阴阳"理论整体论思想在中医学脏腑概念形成中的作用和地位。方法:27只SD雌性大鼠,分成高氧组、低氧组、正常组,每组9只。正常组正常饲养;低氧组(10±1)%低氧刺激,高氧组(40±3)%高氧刺激,2组均每天刺激8 h,共7 d。用放射免疫分析的方法分别检测3组大鼠肺、回肠、结肠以及空肠的VIP、CCK、SP含量变化。结果:正常组:肺与空肠组织上的血管活性肠肽、P物质、胆囊收缩素的表达有显著差异,而回肠、结肠组织没有。高氧组:肺与空肠上的血管活性肠肽、P物质的表达具有显著差异,而回肠、结肠组织没有;肺与回肠、结肠、空肠组织上的胆囊收缩素表达比较,差异无统计学意义。低氧组:肺与空肠组织上的血管活性肠肽、胆囊收缩素表达比较有统计学意义,而回肠、结肠组织上没有;空肠、回肠组织上的p物质表达有统计学意义,而结肠组织上没有。说明变化与外界环境有关。结论:在生理状态下,肺、回肠、结肠这三者之间的联系密切。血管活性肠肽、P物质及胆囊收缩素这3种物质是肺与大肠之间机能相关的物质基础,其变化与外环境相关,这为中医学《黄帝内经》"四时五藏阴阳"理论的研究提供了生物医学的依据。Objective： To discuss the scientific connotation of visceral manifestation of TCM, and the role and status of the “yin-yang of the four seasons and the five viscera” theoretical holistic theory in the formation of the TCM concept of viscera, from the relationship between the lungs and the large intestine, through comparing the expression of vasoactive intestinal peptide （VIP）, cholecystokinin （CCK）, and substance P （SP） in lungs with jejunum, ileum, and colon tissues under different oxygenation index conditions. Methods： Twenty-seven SD female rats were divided into hyperoxia group, hypoxic group and physiological group with 9 rats in each group. The hypoxic group was given （10±1）% oxygen concentration for 8 hours per day, and the hyperoxia group was given （40±3）% oxygen concentration for 8 hours per day. Both groups were fed for 7 days. The contents of VIP, CCK and SP in lung, jejunum, ileum, and colon of rats in each group were determined by radioimmunoassay. Results： In the normal group, the expression of VIP, CCK and SP in lung was significantly different with jejunum, and no differences with ileum and colon. Hypoxia group： Compared with the lungs, the VIP and CCK in the jejunum were significantly different, but there was no difference in ileum and colon; the SP in the jejunum and ileum was significantly different, but there was no difference in colon. Hyperoxia group： compared with the lungs, VIP, SP on the jejunum had significant differences, but no differences on the ileum and colon; CCK in lung and jejunum, ileum, colon was not significantly different. The result explained that changes were related to the external environment. Conclusion： Under physiological conditions, the lung, colon, and ileum are the most closely related to each other. It can be assumed that the lung has a close relationship with the large intestine. At the same time, three brain-intestinal peptides, VIP, CCK and SP, are the material basis for the functional relationship between the lungs and the

关 键 词：含氧量 表达差异 肺合大肠 血管活性肠肽 胆囊收缩素 P物质 

分 类 号：R338[医药卫生—人体生理学]