一株耐盐植物内生真菌的筛选与鉴定  

The Screening and Identification on an Endophytic Fungi of Halotolerant

作　　者：孙海彦[1,2] 尹慧祥 陈惠琴[1] 王琪 Marilyn Roossinck[3] Regina S Redman[4] Rusty Rodriguez 包晓东 SUN Hai-yan;YIN Hui-xiang;CHEN Hui-qin(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Hainan Key Laboratory of Tropical Microbe Resources,Haikou,Hainan 57101)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571011 [2]海南省热带微生物资源重点实验室,海南海口571011 [3]宾夕法尼亚州立大学,美国16802 [4]华盛顿大学,美国98195

出　　处：《安徽农业科学》2018年第19期108-110,共3页Journal of Anhui Agricultural Sciences

基　　金：海南省国际合作项目(KJHZ2015-21);国家木薯产业技术体系岗位科学家项目(CARS-11-HNSHY);中国热带农业科学院基本科研业务费专项(1630052016007;1630052016008)

摘　　要：[目的]研究盐田原生态系统中的耐盐植物内生真菌。[方法]对38株从海南省莺歌海盐场和东方盐场分离的菌株进行耐盐生长测试。[结果]发现了1株在8%Na Cl浓度下生长较好的真菌,并根据其形态特征,ITS扩增序列聚类进化树分析,显示其分类地位为黑酵母菌(Hortaea werneckii)。经过高效液相色谱分析其代谢产物,发现该真菌在0、2%、4%、8%Na Cl浓度下的发酵产物均有所不同。[结论]盐田生境中存在特殊的植物内生真菌区系,其中黑酵母菌Hortaea werneckii具有一定代表性,有深入研究的价值。[Objective]To study the endophytic fungi of halotolerant in the original ecosystem of saltern. [Method]Salt stress tests were conducted on the endophytic fungi isolated from Yingehai Saltern and Dongfang Saltern in Hainan Province,to discover the halotolerant fungi among them. [Result]One isolate was further identified according to morphological and ITS sequence-based phylogenic analyses,which suggested it belongs to Hortaea werneckii,a black yeast species,in taxonomy. The ferment substance under 0,2%,4%,8% of sodium chloride of this isolate was studied with HPLC finger printing approach,and differential metabolites according to salt concentrations were observed. [Conclusion]The solar salterns may be occupied by idiographic microflora of endophytes,included Hortaea werneckii observed in this study,which maybe representative of further studies.

关 键 词：盐田 黑酵母 聚类进化树 高效液相色谱 

分 类 号：S182[农业科学—农业基础科学]