高丹草及其亲本种子胚差异蛋白质组学分析  

Differential proteomics analysis of the embryo in sorghum-sudangrass hybrid and its parents

作　　者：薛春雷[1] 逯晓萍[1] 韩平安 张坤明 张瑞霞 董婧[1] XUE Chunlei;LU Xiaoping;HAN Pingan;ZHANG Kunming;ZHANG Ruixia;DONG Jing(College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019,China;Biological Research Center,Inner Mongolia Academy of Agriculture and Animal Husbandry Science,Hohhot 010020,China;Hohhot Seed Management Station,Hohhot 010019,China)

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古农牧业科学院生物研究中心,呼和浩特010020 [3]呼和浩特市种子管理站,呼和浩特010019

出　　处：《科技导报》2018年第14期88-98,共11页Science & Technology Review

基　　金：国家自然科学基金项目(31160302,31460375);呼和浩特市科技计划项目(2012-重-计-8-2)

摘　　要：高丹草是综合高粱和苏丹草双亲优良性状的一年生禾本科饲草,其杂种优势特别明显,但杂种优势形成机理还不明确。为了揭示高丹草杂种优势形成机理,本研究以杂种高丹草及其亲本的成熟胚为试材,利用Label free结合质谱技术,采用生物信息学分析方法在蛋白质组学水平进行研究。研究结果鉴定出差异蛋白124个,其中加性积累蛋白48个,占差异蛋白总数的38.71%,加性积累蛋白中上调蛋白19个,下调蛋白29个。鉴定出非加性积累蛋白为76个,占差异蛋白总数的61.29%,非加性积累蛋白的表达模式以超高亲表达所占比例最大（29个）,其次是偏高亲表达模式（18个）,偏低亲表达模式次之（14个）,另外还有超低亲表达模式（10个）,以及不属于该四种表达模式蛋白5个,因此,加性积累蛋白在高丹草成熟胚杂种优势形成上起主导作用。加性与非加性积累蛋白涉及多个功能组,主要是胁迫响应、碳水化合物代谢、转录调控、发育调控、信号转导、蛋白质代谢等功能类别。Sorghum-sudangrass hybrids are annual gramineous forage crops with intergrated excellent parental traits. Their heterosis is particularly prominent, but the molecular mechanism of the heterosis is not yet very clear. To reveal the molecular mechanism, the mature embryos of sorghum-sudangrass hybrids and their parents are analyzed by the Label free mass spectrometry and the bioinformatics methods based on proteomics in this study. 124 differentially expressed proteins are identified, among which 48 are additive accumulation proteins, accounting for 38.71% of the total proteins. 19 and 29 of them are up-regulated proteins and down-regulated proteins, respectively. 76 of them are non-additive accumulation proteins, accounting for 61.29% of the total proteins. 29 proteins are of the above-high-parent expression, with the greatest proportion among the non-additive accumulation expression patterns. Followed by the high-parent expression patterns（18 proteins）, the low-parent expression patterns （14 proteins）, the below-low-parent expression patterns（10 proteins）. Besides, there are 5 proteins which do not belong to the above four kinds of protein expression patterns. Therefore, non-additive proteins play especially dominant roles in the heterosis of the mature embryo of sorghum-sudangrass hybrids. Additive and non-additive accumulation proteins cover multiple functional groups, which involve the stress response, the carbohydrate metabolism, the transcriptional regulation, thedevelopment regulation, the signal transduction, the protein metabolism and others.

关 键 词：高丹草 成熟胚 蛋白质组 LABEL free 质谱分析 

分 类 号：S544.9[农业科学—作物学]