rhBMP-2/nano-HAP/NBCA可塑性人工骨的力学性能及体外降解性能研究  

The Study of Mechanical Properties and Degradation Performance of rhBMP-2/nano-HAP/NBCA Composite in vitro

作　　者：叶山山 何春耒[2] 徐景婷 高辉[2] 徐房添[2] YE Shah-shan;HE Chun-lei;XUJing-ting;GAO Hui;XU Fang-tian(Post Graduate Student,Grade 2015;The First Affiliated Hospital of Gannan Medical University;Undergraduate Student,Grade 2013,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院 [2]赣南医学院第一附属医院骨科,江西赣州341000

出　　处：《赣南医学院学报》2018年第5期436-442,共7页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省高等学校科技落地计划项目(编号:KJLD13084);江西省教育厅青年基金项目(编号:GJJ160969)

摘　　要：目的:制备不同组份比的rhBMP-2/nano-HAP/NBCA可塑性人工骨复合物,探究其体外生物力学、降解性能,选出复合物最佳的V_(rhBMP-2/nano-HAP)占比。方法:冷冻干燥法制备_(rhBMP-2/nano-HAP)复合物,低温混合法制备不同组份比的rhBMP-2/nano-HA/NBCA活性复合物。以V_(rhBMP-2/nano-HAP)占比不同将各组份比复合物分为:0%组,5%组,10%组,15%组,20%组,25%组,30%组,35%组,40%组,45%组,50%组,55%组,相互对照,研究不同组份比复合材料的粘结性能、压缩应变及体外降解特点。结果:对数据最优分箱离散化分组后统计分析,复合物中,V_(rhBMP-2/nano-HAP)占比在(0%、5%、10%、15%、20%)时,粘结的小骨段抗弯强度在(6.2±2.5)MPa^(11.1±5.0)MPa之间,保持了最高的粘结力;复合材料V_(rhBMP-2/nano-HAP)占比为(20%、25%、30%、35%)时,压缩应变为(68.6±7.8)%,具有最大的压缩应变性能。统计各时间点各组份比复合物剩余质量百分比进行分析,作曲线拟合同时根据曲线公式估计其在DMEM中的降解终点,V_(rhBMP-2/nano-HAP)占比在0%~25%的降解速率与V_(rhBMP-2/nano-HAP)占比正相关,V_(rhBMP-2/nano-HAP)占比为20%的复合物估计降解终点为第97天,处于骨折愈合时间84~168 d之内。结论:当V_(rhBMP-2/nano-HAP)占比为20%保持了最佳的粘结性,具有良好的最大压缩应变性能,估计的完全降解时间处于骨折愈合时间区间内,是作为研究复合材料生物活性的最佳组份比。Objective： Different proportions of ingredients of rhBMP-2/nano-HAP/NBCA artificial bone composite were prepared to explore their biomechanical and degradation properties in vitro,and the optimal proportion of V（rhBMP-2/nano-HAP） was selected. Methods： Rh BMP-2/nano-HAP composite were prepared by freeze-dryer,and rh BMP-2/nano-HA/NBCA composite with different composition proportions were prepared by low temperature mixing. The composite were divided by different of ingredients into： 0% group,5% group,10% group,15% group,20% group,25% group,30% group,35% group,40% group,45% group,50% group,and 55% group,we were compared them with each others to research the adhesive properties,compressive strain,and degradation characteristics of the composite materials in vitro.Results： After statistical analysis of the data,when the proportions of V（rhBMP-2/nano-HAP）in the composite were（0%,5%,10%,15%,20%）,the flexural strength of the bonded small bone segments were between（6. 2 ± 2. 5） MPa ^（11. 1± 5. 0） MPa,maintaining the highest adhesive property; When the proportions of composite V（rhBMP-2/nano-HAP）were（20%,25%,30%,35%）,the compressive strain was（68. 6 ± 7. 8） %,which had the optimum compressive strain. Count the composite with different compositional proportions remaining mass percentage in the every time points and analysis,doing curve fitting and estimating its degradation end time points in DMEM according to the curve formulation. The degradation rate of composite with different proportion of V（rhBMP-2/nano-HAP）between 0% ~ 25% was positively related to the proportion of V（rhBMP-2/nano-HAP）. The estimated degradation end point of the composite with 20% V（rhBMP-2/nano-HAP）proportion was 97 days,within 84 ~ 168 d of fracture healing time. Conclusion： When the proportion of V（rhBMP-2/nano-HAP）is 20%,the best adhesion is maintained,and the maximum compressive strain performance is suitable,and the degradation characteristics are within the fracture healing time ra

关 键 词：生物材料 骨形态发生蛋白 羟基磷灰石 氰基丙烯酸正丁酯 力学性能 降解性能 

分 类 号：R318.01[医药卫生—生物医学工程]