模拟微重力对大鼠甲状腺滤泡上皮细胞生长特性的影响  

Effects of the rotary cell culture system-simulated microgravity on growth features of thyroid follicular epithelial cells in rats

作　　者：张倍宁 林晶晶 姜楠 闫洪锋 孙宏伟[2] 徐冰心[3] 周金莲[4] 杨鹤鸣[2] 崔彦[1] Zhang Beining;Lin Jingjing;Jiang Nan;Yah Hongfeng;Sun Hongwei;Xu Bingxin;Zhou Jinlian;Yang Heming;Cui Yan(Department of General Surgery,306 Hospital of PLA,Anhui Medical University,Beijing 100101,China)

机构地区：[1]安徽医科大学解放军第306医院临床学院普通外科,北京100101 [2]解放军第306医院普通外科 [3]解放军第306医院特种医学实验研究中心 [4]解放军第306医院病理科

出　　处：《中华内分泌代谢杂志》2018年第7期598-601,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：全军试验技术研究计划重点项目（SYFD1500128）

摘　　要：建立模拟微重力的细胞培养系统，将大鼠甲状腺滤泡上皮细胞（FRTL-5）分为模拟微重力组（SMG）和正常重力对照组（NG），经6、12、24、36 h培养后收集2组细胞，利用MTT法检测细胞增殖，流式细胞仪技术检测细胞周期，激光共聚焦显微镜和荧光素FITC标记技术观察细胞微丝结构形态变化。MTT检测结果显示，与NG组相比，SMG组FRTL-5细胞经6、12、24、36 h培养后，各时相细胞增殖均明显抑制（P〈0.05），其中24 h最为明显。流式细胞仪检测结果显示，与NG组相比，FRTL-5细胞微重力培养6、12、24、36 h后G1期细胞比例显著增高（P〈0.05）；除6 h外，S期细胞比例明显降低；而各时相的G2/M期细胞比例表现为模拟失重早期（6～12 h）降低，其中12 h出现低谷值，24 h一过性显著增高。激光共聚焦显微镜观察发现模拟微重力培养36 h后，细胞微丝骨架局部解聚，张力纤维减少，结构和排列紊乱，细胞伪足少见，细胞形状呈不规则。The rotary cell culture system（RCCS）was used to simulate the microgravity environment, and FRTL-5 cells were divided into simulated microgravity group（SMG）and normal gravity group（NG）. FRTL-5 cells were harvested after treatment for 6, 12, 24, and 36 h, the cell viability was measured by MTT assay, and the cells cycles were detected by flow cytometry. The ultrastructure of FRTL-5 cells was observed under laser confocal microscope with FITC-labeled technique. The MTT assay showed that the proliferation of FRTL-5 cells was significantly inhibited after RCCS treatment for 6, 12, 24, and 36h compared with NG（P〈0.05）, in which the most obvious effect was observed at 24h. The flow cytometry showed that the proportion of FRTL-5 cells at G1 stage in RCCS group was increased significantly after 6, 12, 24, and 36h compared with NG（P〈0.05）, while the proportion of FRTL-5 cells at S stage was decreased significantly（P〈0.05）except that cultured with RCCS for 6 h. The proportion of FRTL-5 cells at G2/M stage was decreased in early phase（6-12 hours）of RCCS culture, with the lowest at 12h and transient increase at 24h of RCCS culture. The laser confocal microscope revealed that there were local microfilament depolymerization, tension fibers decrease, structure disorder, cellular pseudopodia reduction, and irregular shape among FITC-labeled FRTL-5 cells cultured with RCCS for 36h.

关 键 词：模拟微重力 甲状腺滤泡上皮细胞 FRTL-5细胞 增殖 微丝 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]