绝经后女性乳腺癌与体内IGFBP2、FGA表达上调的关系及意义  

Association of Breast Cancer Up-regulated Expression of IGFBP2 and FGA with in Postmenopausal Women

作　　者：热孜亚·肉孜阿洪 迪拉热·力迪甫 迪丽米娜·伊拉木[3] 古扎力努尔买提沙 Abulizi Abudula ROUZIAHONG Reziya;LIDIFU Dilare;YILAMU Dilimina;MAITISHA Guzhalinuer;ABUDULA Abulizi(Department of Biochemistry and Molecular Biology,College of Basic Medicine,Xinjiang Medical University,Ururnqi,830011,China;Experimental Center of Morphology,College of Basic Medicine,Xinjiang Medical University,Urumqi,830011,China;Department of Breast Surgery,The First Affiliated Hospital of Xinjiang Medical University,Uru- mqi,830054,China;Research Center,Xinjiang Medical University,Urumqi,830011,China)

机构地区：[1]新疆医科大学基础医学院生物化学与分子生物学教研室,乌鲁木齐市830011 [2]新疆医科大学基础医学院形态学中心,乌鲁木齐市830011 [3]新疆医科大学第一附属医院乳腺外科,乌鲁木齐市830001 [4]新疆医科大学中心实验室,乌鲁木齐市830011

出　　处：《医学分子生物学杂志》2018年第4期227-231,共5页Journal of Medical Molecular Biology

基　　金：新疆维吾尔自治区高技术研究发展计划（No.201517105）

摘　　要：目的对乳腺癌患者外周血标本进行蛋白质水平筛查分析，明确女性乳腺癌发生及绝经前后与IGFBP-2和FGA表达调控的关系。为建立乳腺癌早期预警及临床治疗疗效评估指标提供依据。方法收集病理确诊为乳腺癌的患者和健康志愿者外周血标本共85例，其中绝经后患者29例，未绝经患者36例，正常对照20例。采用酶联免疫吸附试验（ELISA）方法，检测外周血（血浆）胰岛素样生长因子结合蛋白2（ insulin-like growth factor binding protein 2, IGFBP2）和纤维蛋白原α链（ fibrinogen alpha chain, FGA）含量。结果绝经后乳腺癌患者体内IGFBP-2含量升高，与未绝经患者和正常对照有显著差异（P〈0．05）。正常对照组患者血浆FGA含量低于未绝经及绝经后乳腺癌患者（P〈0．05）。未绝经乳腺癌与绝经后乳腺癌患者之间，FGA含量也存在显著差异（P〈0．05）。受信者操作特性（ receiver operating characteristic, ROC）曲线分析显示，血浆IGFBP-2和FGA表达水平均有较高的灵敏度和特异性。结论绝经后和未绝经乳腺癌患者伴有血浆IGFBP-2与FGA含量升高，可能与乳腺癌IGFBP-2、FGA蛋白质的表达调控异常存在内在联系，此为进一步揭示乳腺癌发病机制、早期预警和临床治疗疗效评估提供依据。Objective s： The protein levels of peripheral blood from patients with breast canc- er were screened and analyzed, to determine the relationship between breast cancer and the expres- sion of IGFBP-2 and FGA of post-menopausal and non-menopausal women, and provide basis for the establishment of early warning of breast cancer and evaluation index of clinical treatment effiea-cy. Methods A total of 85 peripheral blood samples were collected from pathologically diagnosed breast cancer patients and healthy volunteers, including 29 cases of postmenopausal patients, 36 cases of non-menopausal patients, and 20 cases of normal controls. The levels of insulin-like growth factor binding protein 2 （IGFBP2） and fibrinogen alpha chain （FGA） in peripheral blood （plas- ma） were determined by enzyme-linked immunosorbent assay （ELISA） . Results As compared with the normal control group, the plasma level of IGFBP-2 was increased significantly in postmeno- pausal breast cancer group （P 〈 0. 05 ） . However, there was no difference in plasma IGFBP-2 lev- el between the non-menopausal breast cancer group and the normal control group （P 〉 0.05 ） . There was also a significant difference in plasma IGFBP-2 level between non-menopausal women with breast cancer and postmenopausal women with breast cancer （P 〈 0.05 ） . Plasma FGA level was lower in the normal control group than in the non-menopausal and postmenopausal breast cancer patients （ P 〈0. 05 ） . There was also a significant difference in FGA content between non-menopa- usal women with breast cancer and postmenopausal women with breast cancer （P 〈 0.05） . The Receiver Operating Characteristic （ROC） curve analysis showed that the use of ELISA to determine IGFBP-2 and FGA expression levels had higher sensitivity and specificity. Conclusion The occur- rence of female breast cancer is related to abnormal levels of IGFBP-2 and FGAin vh^o. The increase of plasma IGFBP-2 and FGA levels might be associated with breast cancer in post-menopa

关 键 词：乳腺癌 胰岛素样生长因子结合蛋白2 纤维蛋白原d链 外周血 酶联免疫吸附法 

分 类 号：R392.11[医药卫生—免疫学]