基于UPLC-Q-TOF/MS技术的沉香化气丸化学成分分析  

Identification of chemical compositions in Chenxiang Huaqi Pills by UPLC-Q-TOF/MS

作　　者：潘玄玄 宋粉云 李华[2] PAN Xuan-xuan;SONG Fen-yun;LI Hua(College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Institute for Drug Control,Guangzhou 510180,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东省药品检验所,广东广州510180

出　　处：《中草药》2018年第13期2984-2992,共9页Chinese Traditional and Herbal Drugs

基　　金：上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001)

摘　　要：目的采用UPLC-Q-TOF/MS技术对中药复方沉香化气丸中的化学成分进行定性分析。方法采用Phenomenex Kinetex C18色谱柱(100 mm×4.6 mm,2.7μm),以乙睛-0.1%甲酸水溶液为流动相梯度洗脱,体积流量为0.8 mL/min;采用ESI源在正、负离子模式下分别采集数据。根据化合物准确的相对分子质量及二级碎片离子信息,结合参考文献数据及与对照品对照,鉴定沉香化气丸的主要化学成分。结果从沉香化气丸中分离和鉴定出73个化学成分,包括36个黄酮类、16个2-(2-苯乙基)色酮类、7个三萜皂苷类、2个倍半萜内酯及12个其他类成分。结论 UPLC-Q-TOF/MS联用技术为鉴别沉香化气丸中化学成分提供简便、快速、准确的方法,为进一步分析沉香化气丸的物质基础及质量控制提供了新技术方法。Objective To analyze the main chemical constituents of traditional Chinese medicine compound Chenxiang Huaqi Pills by using UPLC-Q-TOF/MS technology. Methods The separation was performed on Phenomenex Kinetex C18 column（100 mm×4.6 mm, 2.7 μm）, and the gradient elution of acetonitrile-0.1% formic acid was used as mobile phase at a flow rate of 0.8 mL/min. The data was collected by the positive and negative ion modes using Q-TOF/MS and ESI source. The main chemical constituents of Chenxiang Huaqi Pills were identified according to the exact molecular mass, the cleavage fragments of MS/MS, the literature data, and the reference control. Results A total of 73 chemical components were separated and identified in Chenxiang Huaqi Pills, including 36 flavonoids, 16 2-（2-phenylethyl） chromones, 7 triterpenoid saponins, 2 sesquiterpene lactones, and 12 other components. Conclusion This study showed that UPLC-Q-TOF/MS technology provided a simple, rapid, and accurate method for the identification of chemical constituents in Chenxiang Huaqi Pills, which provided a new technology method for the pharmacological basis and quality control of Chenxiang Huaqi Pills.

关 键 词：沉香化气丸 UPLC-Q-TOF/MS 黄酮类 2-(2-苯乙基)色酮类 三萜皂苷类 

分 类 号：R284.1[医药卫生—中药学]