失效模式分析和星点设计-效应面法优化熟三七皂苷类成分纯化工艺  

Optimization of purification process of saponins in steamed Panax notoginseng by failure mode and effects analysis and central composite design-response surface methodology

作　　者：胡玉飘 崔秀明[1,2,3] 张泽君 陈丽娟 于未博 熊吟[1,2,3] HU Yu-piao;CUI Xiu-ming;ZHANG Ze-jun;CHEN Li-juan;YU Wei-bo;XIONG Yin(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Yunnan Key Laboratory ofPanax Notoginseng,Kunming University of Science and Technology,Kunming 650500,China;Laboratory of Sustainable Utilization of Panax Notoginseng Resources State Administration of Traditional Chinese Medicine,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]昆明理工大学云南省三七重点实验室,云南昆明650500 [3]昆明理工大学国家中医药管理局三七资源可持续利用实验室,云南昆明650500

出　　处：《中草药》2018年第13期3009-3016,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目:三七"生打熟补"效用关联的物质基础及个体化质控方法研究(81660661)

摘　　要：目的优化熟三七中皂苷类成分的大孔吸附树脂纯化工艺。方法通过失效模式分析(FMEA)筛选纯化工艺的主要影响因素,采用星点设计-效应面法(CCD-RSM),以皂苷类成分的回收率和质量分数为评价指标,对大孔吸附树脂纯化工艺进行优选。实验中以5个主要影响因素(上柱液质量浓度、上样体积、水洗体积、乙醇体积分数、乙醇洗脱体积)考察了熟三七皂苷类成分的纯化工艺。结果以大孔吸附树脂为纯化工艺,优化工艺为当上柱液质量浓度为11.22 mg/mL,上样体积为4.97 BV,水洗体积为2 BV,乙醇体积分数为70%,乙醇洗脱体积为3.31 BV时,可获得最大的皂苷回收率(82.81%)和皂苷质量分数(77.24%)。结论优化的纯化工艺简单、稳定,熟三七皂苷类成分回收率和质量分数均较高,具有一定的实用价值。Objective To optimize the purification technology of saponins in steamed Panax notoginseng with macroporous resin. Methods The main factors affecting the purification process were screened by failure mode and effects analysis（FMEA）. The purification method with macroporous resin was optimized by central combination design-response surface method（CCD-RSM） based on the recovery and purity of saponins. In this experiment, the concentration of sample solution, loading volume, washing volume, ethanol concentration, and ethanol elution volume were used to investigate the purification of saponins in steamed P. notoginseng. Results The optimized purification process with macroporous resin was as follows： maximum recovery（82.81%） and purity（77.24%） of saponins were obtained with the concentration of saponin solution of 11.22 mg/mL, loading volume of 4.97 BV, washing volume of 2 BV, ethanol concentration of 70%, and ethanol elution volume of 3.31 BV. Conclusion The optimized purification process based on FMEA and CCD-RSM is convenient and stable, with high recovery and purity of saponins, which has a certain practical value.

关 键 词：熟三七 皂苷类成分 大孔吸附树脂 失效模式分析 星点设计-效应面法 纯化工艺 回收率 

分 类 号：R284.2[医药卫生—中药学]