桂花种子环烯醚萜苷的提取工艺与抗血栓活性研究  

Iridoid glycoside extraction technology and anti-thrombotic activity of Osmanthus fragrans seeds

作　　者：李桂华 王苗苗 贺魏 刘洁 张蝶 刘嘉琪 唐伟卓 LI Gui-hua;WANG Miao-miao;HE Wei;LIU Jie;ZHANG Die;LIU Jia-qi;TANG Wei-zhuo(College of Biological and Environmental Engineering,Changsha University,Changsha 410022,China)

机构地区：[1]长沙学院生物与环境工程学院,湖南长沙410022

出　　处：《中草药》2018年第13期3033-3041,共9页Chinese Traditional and Herbal Drugs

基　　金：2017年湖南省大学生研究性学习和创新性实验计划项目(775);国家自然科学基金资助项目(41506149);长沙市科技计划项目(k1705046)

摘　　要：目的优选桂花4大品种种子中2种主要环烯醚萜苷类成分(特女贞苷和女贞苷G13)的提取工艺并对桂花种子进行抗血栓活性评价。方法采用L_9(3~4)正交设计,从乙醇体积分数、料液比和提取时间3个方面考察超声、回流、微波不同提取方法对桂花种子中2种成分的提取效果。以提取率、成分质量及总峰面积为指标进行综合加权分析,确定最佳的提取方法和提取工艺。建立斑马鱼抗血栓模型评价桂花种子的抗血栓活性。结果 3种提取方法中,微波提取效果最好,最佳提取工艺为乙醇体积分数55%、料液比1∶10、提取时间15 min。HPLC分析表明桂花4大品种种子的化学成分组成基本一致。斑马鱼抗血栓活性结果显示,桂花种子提取物的血栓抑制作用不明显,在高浓度剂量下易导致心包水肿。结论考察了桂花4大品种种子中2种环烯醚萜苷的提取工艺,同时初步研究了银桂品种种子水提物的抗血栓活性,为桂花子这种废弃资源的二次开发和利用提供了参考。Objective To optimize the extraction technology of two major iridoid glucosides（specnuezhenide and nuezhenoside G13） from four kinds of Osmanthus fragrans（OF） seeds, and to evaluate the anti-thrombotic activity of OF seeds. Methods The orthogonal-test experiment was employed to optimize the parameters including ethanol concentration, liquid-material ratio, and extraction time for three extraction methods（ultrasonic extraction, reflux extraction, and microwave extraction）. The extraction yield, content, and total peak area of iridoid glucosides were selected for weighted analysis to determine the best extraction method and technology. Additionally, an anti-thrombotic zebra fish model was established for biological evaluation of OF seeds. Results Microwave extraction was the best method for iridoid glucosides extraction with the optimal conditions of ethanol concentration 55%, material-liquid ratio 1∶10, and microwave time 15 min. HPLC analysis showed that there was no significant difference in chemical composition among the four kinds of OF seeds. In zebra fish biological screening model, OF seeds displayed a weak inhibitory effect on the growth of thrombus and exhibited a pericardial edema effect in high dose-treated group. Conclusion In this paper, extraction technology of two iridoid glucosides from four different kinds of OF seeds and preliminary anti-thrombotic activity evaluation of OF seeds were investigated. These results can provide the reference for further development and utilization of the agricultural waste of OF seeds.

关 键 词：桂花种子 特女贞苷 女贞苷G13 提取工艺 抗血栓活性 正交设计 斑马鱼 抗血栓模型 

分 类 号：R284.2[医药卫生—中药学]