贵州不同产地粗毛淫羊藿药用植物遗传多样性及亲缘关系的ISSR分析  

ISSR analysis of genetic diversity and relationship among medicinal plants of Epimedium acuminatum from different habitats in Guizhou Province

作　　者：关萍[1] 彭昌琴 李牡丹 杨鹏[1] 陈兴银 刘鑫 石建明[1] SHI Jian-ming;LI Mu-dan;YANG Peng;CHEN Xing-yin;PENG Chang-qin;LIU Xin;GUAN Ping(College of Life Sciences,Guizhou University,Guiyang 550025,China;Guangdong Institute of National Basic Education Development and Research,Guangzhou 511400,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]广东省国基教育发展研究院,广东广州511400

出　　处：《中草药》2018年第13期3122-3126,共5页Chinese Traditional and Herbal Drugs

基　　金：贵州省社会发展攻关课题(201303137);中央高校基本业务费一般项目(XDJK2016C006)

摘　　要：目的对贵州不同产地粗毛淫羊藿样品的遗传多样性和亲缘关系进行分析。方法利用Pop Gene 32软件及NTsys2.10e软件分析贵州5个不同产地粗毛淫羊藿的遗传多样性及亲缘关系,并根据UPGMA法,构建亲缘关系树状图。结果从100个随机引物中筛选出13条多态性稳定、条带清晰的引物,结果表明,共扩增出211个位点,从物种水平上看,共有205个多态性位点,多态性百分率(PPB)为97.16%,Shannon’s信息指数(I)为0.455 7,Nei’s基因多样性指数(H_e)为0.297 3,有效等位基因数(N_e)为1.490 2,5个居群间总的遗传多样性(H_t)为0.297 3,而居群内的遗传多样性(H_s)为0.207 5,表明粗毛淫羊藿的遗传变异主要存在于群体内,种群内的遗传分化大于种群间。利用UPGMA法构建的居群遗传距离聚类树,表明,5个居群被分为2支,其中来自安顺、花溪、高坡的3个居群聚在一支,来自龙里和雷山的2个居群共同聚为一支。结论 ISSR分子标记技术可以用于贵州不同产地的粗毛淫羊藿植物的遗传多样性和亲缘关系分析。Objective To analyze the genetic diversity and relationship of Epimedium acuminatum from different habitats in Guizhou Province. Methods Pop Gene 32 software and NTsys2.10 e software was used to analyze the genetic diversity and phylogenetic relationship of five different habitats in Guizhou Province, and phylogenetic tree was constructed according to the UPGMA method. Results A total of 13 polymorphic and clear bands were screened from 100 random primers showed that amplified 211 bands, of which 205 were polymorphic from the level of species, the percentage of polymorphic bands（PPB） was 97.16%. The Shannon＇s information index（I） was 0.455 7; Nei＇s gene diversity index（H_e） was 0.297 3; The effective number（N_e） of alleles was 1.490 2; The total genetic diversity（H_t） of five populations was 0.297 3, while the population genetic diversity within the group（H_s） was 0.207 5, indicated that the genetic variation mainly existed within populations, genetic differentiation within population than that of population between. The population genetic distance clustering was built by UPGMA. The results showed that five populations were divided into two branches, three populations from Anshun, Huaxi, and Gaopo clustered into one branch, and the other two populations fromLongli and Leishan populations clustered into one branch. Conclusion ISSR molecular marker technique can be used to analyze the genetic diversity and phylogenetic relationship of Epimedium acuminatum from different habitats in Guizhou Province.

关 键 词：粗毛淫羊藿 ISSR 遗传多样性 亲缘关系 UPGMA法 

分 类 号：R282.12[医药卫生—中药学]