虾脊兰属11种药用植物rDNA ITS的克隆与序列分析  

Cloning and analysis of rDNA ITS sequences from 11 medical plants in Calanthe

作　　者：蒋明[1] 吴棣飞 鲍洪华 贺蔡明[1] 朱欣[1] 吕奕[1] 蒋晓颖[1] JIANG Ming;WU Di-fei;BAO Hong-hua;HE Cai-ming;ZHU Xin;LV Yi;JIANG Xiao-ying(College of Life Science,Taizhou University,Jiaojiang 318000,China;Department of Park Management ofWenzhou,Wenzhou 325000,China;Luqiao Environmental Monitoring Station of Taizhou,Luqiao 318050,China)

机构地区：[1]台州学院生命科学学院,浙江椒江318000 [2]温州市公园管理处,浙江温州325000 [3]台州市路桥区环境监测站,浙江路桥318050

出　　处：《中草药》2018年第14期3369-3375,共7页Chinese Traditional and Herbal Drugs

基　　金：台州市211人才工程经费(2012年度)

摘　　要：目的通过比对11种虾脊兰属药用植物的rDNA ITS序列,为该属植物的分子鉴定奠定基础。方法以叶片基因组DNA为材料,利用PCR法克隆虾脊兰属植物的ITS序列,利用Clustal、Gen Doc和Mega等软件进行生物信息学分析。结果 11种虾脊兰属植物的ITS全长为652~661 bp,其中ITS1为228~230 bp,ITS2为256~264 bp,5.8 S的长度则完全一致,均为167 bp;ITS1和ITS2分别有44与46个变异位点,其中信息位点数为22和24个,而5.8 S中没有检测到变异位点和信息位点;系统发育分析结果表明,11种虾脊兰属植物之间的遗传距离为0.002~0.081,在发育树上可分为3组,I~Ⅲ组的物种数量分别为5、4和2。结论 11种虾脊兰属植物的ITS序列具有丰富的信息位点,可用于这些药用植物的分子鉴定。Objective To provide evidences for molecular identification, sequence comparisons of 11 rDNA ITS in Calanthe plants were performed. Methods ITS sequences of Calanthe plants were isolated by using Polymerase Chain Reaction（PCR） method, and then analyzed by software namely Clustal, Gen Doc, and Mega. Results The full ITS sequences of 11 Calanthe plants ranged from 652 to 661 bp; ITS1 and ITS2 sequences were 228—230 bp and 256—264 bp in length, respectively, while their 5.8 S sequences were identical with a length of 167 bp. The variable sites in both ITS1 and ITS2 were 44 and 46, respectively. Parsimony information sites of ITS1 and ITS2 were 22 and 24, however, there was no variable and information sites indentified in 5.8 S sequences. Genetic distance among 11 Calanthe species varied from 0.002 to 0.081, and they were divided into three groups in a phylogenetic tree with species numbers of 5, 4, and 2 in I—Ⅲ groups, respectively. Conclusion The ITS sequences of 11 Calanthe plants were rich in information sites, which could be applied for molecular identification.

关 键 词：虾脊兰属 RDNA ITS 克隆 序列分析 

分 类 号：R282.12[医药卫生—中药学]