HLA-DR^(-/low)CD33^+CD11b^+CD15^+CD14^-多型核粒细胞在子宫内膜异位症中的作用  

The significance of HLA-DR^(-/low)CD33^+CD11b^+CD15^+CD14^- polymorphonuclear neutrophils in endometriosis

作　　者：陈海文 李锦城[2] 雷爱华[1] CHEN Hai-wen;LI Jin-cheng;LEI Ai-hua(Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,Guangdong 510080;Lingnan Hospital,Department of Laboratory Medicine,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510630,China)

机构地区：[1]中山大学中山医学院,广东广州510080 [2]中山大学附属第三医院岭南医院检验科,广东广州510630

出　　处：《热带医学杂志》2018年第7期850-854,共5页Journal of Tropical Medicine

基　　金：中国博士后科学基金(2017M612809)

摘　　要：目的探讨HLA-DR^(-/low)CD33^+CD11b^+CD15^+CD14-多型核粒细胞(PMN,简称CD15^+PMN)在子宫内膜异位症(Endo)中的临床意义。方法用流式细胞术检测正常体检者(HC组,n=7)、不孕症患者(Control组,n=25)及Endo患者(Endo组,n=26)外周血单核细胞(PBMCs)中CD15^+PMN的水平并测定其体外免疫抑制功能,同时检测Control组和Endo组腹水单个核细胞(PFMCs)中CD15^+PMN的水平。结果与HC组和Control组相比,Endo组PBMCs中CD15^+PMN的水平明显升高;与Control组相比,Endo组PFMCs中该细胞群的水平也明显上升,且Endo组患者晚期阶段PBMCs和PFMCs中CD15^+PMN的水平明显高于早期阶段,差异均有统计学意义(P<0.05)。体外T细胞增殖实验发现Endo组患者PBMCs中CD15^+PMN不具有抑制T细胞增殖的能力。结论 Endo患者PBMCs和PFMCs中存在异常增多的CD15^+PMN,提示CD15^+PMN可能参与子宫内膜异位症的发生和发展。Objective To explore the clinical significance of HLA-DR（-/low）CD33＋CD11 b＋CD15＋CD14-polymorphonuclearneutrophils（PMN）in endometriosis（Endo）.Methods Seven healthy controls,twenty-five infertile patients and twentysixendometriosis patients were enrolled in this study.The levels of HLA-DR-/lowCD33＋CD11 b＋CD15＋CD14-cells in PBMCs andPFMCs were analyzed by flow cytometry,and the effect of HLA-DR-/lowCD33＋CD11 b＋CD15＋CD14-cells on T cell proliferationwas determined by CFSE dilution.Results The proportion and absolute number of CD15＋PMN in PBMCs and PFMCs ofendometriosis patients were significantly increased compared with controls（P〈0.05）.There was also significantly increasedin CD15＋PMN level of PBMCs and PFMCs at the stage Ⅲ/Ⅳ of endometriosis.Depletion of Endo-derived CD15＋PMN fromPBMCs did not influence T-cell response.Conclusion We demonstrated that there exited abnormal expansion of CD15＋PMN in PBMCs and PFMCs of patients with endometriosis,suggesting that CD15＋PMN may be involved in the developmentof endometriosis.

关 键 词：子宫内膜异位症 多型核粒细胞 CD15^+PMN 

分 类 号：R711.71[医药卫生—妇产科学]