银杏内酯注射液和银杏内酯ABC对血小板活化因子诱导的家兔血小板聚集作用比较  

Comparison of effects of Ginkgolide Injection and Ginkgolide A, B, and C on platelet aggregation induced by PAF in rabbits

作　　者：季传平 徐露[2] 李慧琴 JI Chuanping;XU Lu;LI Huiqin(Chengdu Baiyu Pharmaceutical Co.Ltd.,Chengdu 610000,China;Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]成都百裕制药股份有限公司,四川成都610000 [2]重庆医科大学生物化学与分子药理学重点实验室,重庆400016

出　　处：《药物评价研究》2018年第7期1174-1178,共5页Drug Evaluation Research

摘　　要：目的观察银杏内酯注射液、银杏内酯ABC以及银杏叶提取物注射液(EGb761)对家兔血小板聚集功能、超微结构及PF-4和β-TG含量的影响比较。方法将日本大耳兔分为对照(生理盐水)组、EGb761(1.02 m L/kg)组、银杏内酯ABC(5.13 mg/kg)组、银杏内酯注射液(1.02 m L/kg,以萜内酯计5.1 mg/kg)组、白果内酯(5.13 mg/kg)组,分别iv相应药物1周。给药结束后,家兔心脏取血,观察PAF诱导下血小板聚集率;电镜观察血小板超微结构;试剂盒法观察PAF诱导下血清中血小板因子-4(PF-4)和β-血小板球蛋白(β-TG)含量。结果与对照组比较,EGb761组、银杏内酯ABC组、银杏内酯注射液组的血小板聚集率均显著降低(P<0.05、0.01),白果内酯组无显著变化;血小板聚集抑制率:银杏内酯注射液(43.76%)>银杏内酯ABC(35.3%)>EGb761(26.52%)>白果内酯(5.48%)。与对照组比较,EGb761、银杏内酯ABC、银杏内酯注射液均能减少聚集型血小板数量,使树突型血小板突起变少变短。与对照组比较,银杏内酯注射液和银杏内酯ABC均使PF-4和β-TG表达显著降低(P<0.01),EGb761仅显著降低β-TG表达(P<0.05)。结论银杏内酯注射液通过PAF途径发挥抗血小板聚集作用,其作用强于银杏内酯ABC,可能是白果内酯发挥了协同增效作用。Objective To observe the effects of Ginkgolide Injection, ginkgolide ABC and ginkgo biloba extract（EGb761） on platelet aggregation function, ultrastructure, and contents of PF-4 and β-TG in rabbits. Methods Rabbits were divided into control（physiological saline） group, EGb761（1.02 mL/kg） group, ginkgolide ABC（5.13 mg/kg） group, Ginkgolide Injection（1.02 mL/kg, as terpene lactones 5.1 mg/kg） group, and bilobalide（5.13 mg/kg） group, respectively. The corresponding drugs were iv injected for one week respectively. After administration, the blood was taken in the heart of rabbits. The platelet aggregation rate induced by platelet activating factor（PAF） was observed, and the ultrastructure of the platelets was observed by electron microscope, and the content of platelet factor-4（PF-4） and beta thromboglobulin（β-TG） in serum induced by PAF was observed by the kit method. Results Compared with control group, the platelet aggregation rates in group EGb761, ginkgolide ABC, and Ginkgolide Injection were significantly decreased（P〈0.05 and 0.01）, and no significant changes were found in the group of bilobalide. The inhibition rate of platelet aggregation was Ginkgolide Injection（43.76%） ginkgolide ABC（35.3%） EGb761（26.52%）bilobalide（5.48%）. Compared with control group, EGb761, ginkgolide ABC and Ginkgolide Injection could reduce the number of aggregated platelets and make dendritic protrusions shorter; Ginkgolide Injection and ginkgolide ABC significantly decreased the expression of PF-4 and β-TG（P〈0.01）, and EGb761 only decreased the expression of β-TG（P〈0.05）. Conclusion Ginkgolide injection plays an anti-platelet aggregation effect through PAF pathway with better effect than ginkgolide ABC, which may be that ginkgolide plays a synergistic effect.

关 键 词：血小板活化因子(PAF) 银杏内酯注射液 银杏内酯ABC 白果内酯 血小板因子-4(PF-4) β-血小板球蛋白(β-TG) 

分 类 号：R962.2[医药卫生—药理学]