Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond  

作　　者：WANG Cuicui ZHANG Kai XIE Jun LIU Qigen YU Deguang WANG auangjun YU Ermeng GONG Wangbao LI Zhifei 王翠翠;张凯;谢骏;刘其根;余德光;王广军;郁二蒙;龚望宝;李志斐(Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences;College of Fisheries and Life Sciences, Shanghai Ocean University)

机构地区：[1]Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China [2]College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Journal of Oceanology and Limnology》2018年第3期913-925,共13页海洋湖沼学报（英文）

基　　金：Supported by the National Key Technology R&D Program of China(Nos.2012BAD25B04,2012BAD25B01);the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-46-17);the National Natural Science Foundation of China(No.31302201);the Pearl River S&T Nova Program of Guangzhou(No.2014J2200088);the Science and Technology Program of Guangzhou(No.201707010311)

摘　　要：This work evaluates the application potential of a new indigenous aerobic denitrifi er, strain Pseudomonas CW-2, isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore, when nitrate and ammonia coexisted, the strain gave priority to assimilating ammonia, and thereafter to denitrifi cation. Under optimal cultivation conditions, citrate and acetate were the carbon resources, C/N was 8, dissolved oxygen was 5.2 mg/L, and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia, nitrite, and phosphates were observed in the treated water as compared with the controls. Meanwhile, phylum-level distributions of the bacterial OTUs revealed that P roteobacteria, Bacteroidetes, Planctomycetes, and N itrospirae continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrifi cation process was weakened under the ef fects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems, according to our evaluations of its denitrifi cation performance.This work evaluates the application potential of a new indigenous aerobic denitrifi er, strain Pseudomonas CW-2, isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore, when nitrate and ammonia coexisted, the strain gave priority to assimilating ammonia, and thereafter to denitrifi cation. Under optimal cultivation conditions, citrate and acetate were the carbon resources, C/N was 8, dissolved oxygen was 5.2 mg/L, and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia, nitrite, and phosphates were observed in the treated water as compared with the controls. Meanwhile, phylum-level distributions of the bacterial OTUs revealed that P roteobacteria, Bacteroidetes, Planctomycetes, and N itrospirae continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrifi cation process was weakened under the ef fects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems, according to our evaluations of its denitrifi cation performance.

关 键 词：aerobic denitrification ammonium removal Micropterus salmoides Pseudomonas CW-2 

分 类 号：S955[农业科学—水产养殖] Q939.1[农业科学—水产科学]