慢性铅暴露对大鼠脑组织PARP1基因mRNA表达影响及与氧化应激的关系  

Effects on expressions of PARP1 gene mRNA and their relationships with oxidative stress in brain tissues of rats induced by chronic lead exposure

作　　者：李炜娟 杨天富 宛明 徐群英 任清风 张中伟 冯建高 肖元梅 LI Wei-Juan;YANG Tian.Fu;WAN Ming(Fuzhou Medical College of Nanchang University,Fuzhou 344000,Jiangxi,Chin)

机构地区：[1]南昌大学抚州医学院,江西抚州344000

出　　处：《中国老年学杂志》2018年第15期3727-3730,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81160342);江西省自然科学基金资助项目(20122BAB205047);江西省教育厅科技项目(GJJ11312);抚州市社会发展指导性科技计划项目(抚科计字[2015]13号)

摘　　要：目的观察饮水铅暴露对大鼠大脑皮质、小脑、海马组织中腺苷酸二磷酸核糖聚合酶(PARP)1基因m RNA表达的影响及其与氧化应激的关系。方法 40只SD大鼠根据体重按随机区组法分为5组(对照组和4个暴露组),每组分别自由饮用去离子水、100、200、400、800 mg/L的醋酸铅溶液,连续染毒60 d后取大脑皮质、小脑和海马,RT-PCR技术检测脑组织PARP1基因m RNA的表达量,并测定脑组织铅、超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-PX)的含量。结果与对照组比较,4个铅暴露组大脑皮质、小脑和海马中PARP1 m RNA表达量显著下降,铅含量显著上升(均P<0.05);最低剂量组大脑皮质及小脑SOD、GSH-PX含量,低剂量组大脑皮质及小脑SOD、GSH-PX含量、海马SOD含量,中、高剂量组大脑皮质、小脑、海马中SOD、GSH-PX含量均显著低于对照组(均P<0.05);最低剂量组海马MDA,低剂量组小脑及海马MDA,中、高剂量组大脑皮质、小脑、海马MDA含量均显著高于对照组(均P<0.05)。随着染铅剂量的升高,各脑组织SOD、GSH-PX含量基本呈逐渐下降趋势,MDA水平基本呈逐渐升高趋势。大脑皮质、小脑、海马PARP1 m RNA表达量均与其组织铅含量及MDA含量呈显著负相关(P<0.05,P<0.01),与SOD含量呈显著正相关(P<0.05);大脑皮质及小脑PARP1 m RNA表达量与GSH-PX含量显著正相关(P<0.05,P<0.01)。结论铅可通过诱导细胞氧化应激影响PARP1基因m RNA表达。Objective To observe the effects of lead exposure through drinking water on the expression of PARP1 mRNA in cerebral cortex, cerebellum and hippocampus of rats and its relationship with oxidative stress. Methods 40 SD rats were divided randomly into five groups which had one control group and four exposure groups according to the weight block design, and drank unlimitedly distilled water con- taining 0, 100,200,400, 800 mg/L lead acetate for 60 days respectively. The expression of PARP1 mRNA in the brain was detected with RT-PCR after separation of the cerebral cortex, cerebellum, and hippocampus. At the same time, lead content in brain tissue and SOD, MDA and GSH-PX were detected. Results Compared with control group, lead exposure groups had lower expression of PARP1 mRNA in the cerebral cortex, hippocampus and cerebelIum（P〈0.05）, and the expression negatively correlated with lead levels in brain tissue and MDA （ r = -0.382, -0. 453, -0.636, all P〈0.05 ; -0.428, -0.571, -0.633, all P〈0.01 ）, while positively correlated with SOD and GSH-PX （ r = 0.486,0. 420,0.637,0.315,0.454,0.331, all P〈0.05 ）. Conclusions Lead can affect the expression of mRNA gene in PARP1 through inducing cell oxidative stress.

关 键 词：腺苷酸二磷酸核糖聚合酶(PARP)1 氧化应激 铅 

分 类 号：R114[医药卫生—卫生毒理学]