CRISPR-dCas9调控基因转录的研究进展  

The Research Progress of CRISPR-dCas9 in Transcriptional Regulation

作　　者：田开仁 薛二淑 宋倩倩 乔建军[1,2,3] 李艳妮 TIAN Kai-ren;XUE Er-shu;SONG Qian-qian;QIAO jian-jun;LI Yan-ni(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Key Laboratory of Systems Bioengineering of Ministry of Education,Tianjin University,Tianjin 300072,China;Syn Bio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学化工学院,天津300072 [2]系统生物工程教育部重点实验室,天津300072 [3]天津化学化工协同创新中心合成生物学平台,天津300072

出　　处：《中国生物工程杂志》2018年第7期94-101,共8页China Biotechnology

基　　金：国家自然科学基金(31570089)资助项目

摘　　要：CRISPR/Cas9系统已不仅仅是一种革命性的基因编辑工具,还能在各种原核和真核生物中调控基因转录。近年来,由CRISPR/Cas9衍生而来的CRISPR-dCas9系统已被用于基因成像、高通量筛选、基因调控、必需基因功能研究及表观遗传调控等多个方向。总结了近年来CRISPRdCas9系统在激活或抑制基因转录、降低脱靶效率、sgRNA与转录强度及特异性之间的联系等方面的研究进展,以期对CRISPR-dCas9系统定向调控基因转录的研究提供参考和帮助,并就其未来可能的改进进行了展望。The CRISPR/Cas9 system is not only a revolutionary tool for gene editing but also regulates gene transcription in various prokaryotic and eukaryotic organisms. In recent years,the system of CRISPR-dCas9 derived from CRISPR/Cas9 has been used in many fields such as gene imaging,high-throughput screening,gene regulation,investigating essential gene function and epigenetic regulation. The recent advances of CRISPR-dCas9 for activating or inhibiting gene transcription, reducing off-targeting efficiency and combing the intrinsic relationship between sgRNA and transcriptional regulation,application in the life science and further upgrading were described.

关 键 词：CRISPR-dCas9 基因调控 转录激活 转录抑制 

分 类 号：Q789[生物学—分子生物学]