Transcriptomic responses to aluminum(Al)stress in maize  

作　　者：XU Li-ming LIU Chan CUI Bao-ming WANG Ning ZHAO Zhuo ZHOU Li-na HUANG Kai-feng DING Jian-zhou DU Han-mei JIANG Wei ZHANG Su-zhi 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Maize in Southwest China,Ministry of Agriculture/Maize Research Institute,Sichuan Agricultural University,Chengdu 611130,P.R.China [2]Crop Research Institute of Binzhou,Binzhou 256600,P.R.China

出　　处：《Journal of Integrative Agriculture》2018年第9期1946-1958,共13页农业科学学报（英文版）

基　　金：supported by the National Basic Research Program of China(973 Program,2014CB138705);the National Natural Science Foundation of China(30800687,31071434);the PhD Programs Foundation of Ministry of Education of China(1.20125103110011)

摘　　要：Aluminum （AI） toxicity is a major factor limiting crop production and plant growth in acid soils. The complex inheritance of AI toxicity and tolerance mechanisms in maize has uncharacterized yet. In this study, the maize inbred line 178 seedlings were treated with 200 μmol L-1 CaCI2＋0 μmol L^-1AICI3 （control） and 200μmol L^-1 CACI2＋60 IJmol L^-1 AICI3 （AI treatment） for I and 6 h, respectively. The experiment was repeated three times. Then a detailed temporal analysis of root gene expression was performed using an Agilent GeneChip with 34 715 genes, only the genes showing more than 2.0-fold difference （P〈0.01） between the control and the AI treatment maize seedlings were analyzed further. Thus, a total of 832 different expression genes, 689 significantly up-regulated and 143 down-regulated, were identified after the seedlings were treated with AI for 6 h. And 60 genes, 59 up-regulated and one down-regulated, were also detected after the seedlings were treated for 1 h. Replicated transcriptome analyses further showed that about 61% of total significantly genes could be annotated based on plant genome resources. Quantitative real-time PCR （qRT-PCT） of some selected candidate genes was used to demonstrate the microarray data, indicating significant differences between the control and AI-treated seedlings. Exposure to AI for 6 h triggered changes in the transcript levels for several genes, which were primarily related to cell wall structure and metabolism, oxidative stress response, membrane transporters, organic acid metabolism, signaling and hormones, and transcription factors, etc. After AI-treated for 1 h, differential abundance of transcripts for several transporters, kinase, and transcription factors were specifically induced. In this study, the diversity of the putative functions of these genes indicates that AI stress for a short stage induced a complex transcriptome changes in maize. These results would further help us to understand rapid and early mechanisms of AI toxicity and tAluminum （AI） toxicity is a major factor limiting crop production and plant growth in acid soils. The complex inheritance of AI toxicity and tolerance mechanisms in maize has uncharacterized yet. In this study, the maize inbred line 178 seedlings were treated with 200 μmol L-1 CaCI2＋0 μmol L^-1AICI3 （control） and 200μmol L^-1 CACI2＋60 IJmol L^-1 AICI3 （AI treatment） for I and 6 h, respectively. The experiment was repeated three times. Then a detailed temporal analysis of root gene expression was performed using an Agilent GeneChip with 34 715 genes, only the genes showing more than 2.0-fold difference （P〈0.01） between the control and the AI treatment maize seedlings were analyzed further. Thus, a total of 832 different expression genes, 689 significantly up-regulated and 143 down-regulated, were identified after the seedlings were treated with AI for 6 h. And 60 genes, 59 up-regulated and one down-regulated, were also detected after the seedlings were treated for 1 h. Replicated transcriptome analyses further showed that about 61% of total significantly genes could be annotated based on plant genome resources. Quantitative real-time PCR （qRT-PCT） of some selected candidate genes was used to demonstrate the microarray data, indicating significant differences between the control and AI-treated seedlings. Exposure to AI for 6 h triggered changes in the transcript levels for several genes, which were primarily related to cell wall structure and metabolism, oxidative stress response, membrane transporters, organic acid metabolism, signaling and hormones, and transcription factors, etc. After AI-treated for 1 h, differential abundance of transcripts for several transporters, kinase, and transcription factors were specifically induced. In this study, the diversity of the putative functions of these genes indicates that AI stress for a short stage induced a complex transcriptome changes in maize. These results would further help us to understand rapid and early mechanisms of AI toxicity and t

关 键 词：ALUMINUM MAIZE MICROARRAY mechanism TRANSCRIPTOME 

分 类 号：S513[农业科学—作物学]