The biotypes and host shifts of cotton-melon aphids Aphis gossypii in northern China  

作　　者：ZHANG Shuai LUO Jun-yu WANG Li WANG Chun-yi LU Li-min ZHANG Li-juan ZHU Xiang-zhen CUI Jin-jie 

机构地区：[1]State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,P.R.China

出　　处：《Journal of Integrative Agriculture》2018年第9期2066-2073,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31572015);the National Special Transgenic Project of China(2016ZX08012-004)

摘　　要：Aphis gossypfi is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear. In this study, the Cytb and 16S gene regions of A. gossypfi collected from 5 common summer hosts and 4 primary hosts were analyzed. A total of 57 haplotypes were obtained from 1 046 individual A. gossyp# sequences. The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites. The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual. A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals. Considering the individual host plant, 5 biotypes were identified. Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races. Type 3 was the most abundant biotype in cotton fields in northern China.Aphis gossypfi is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear. In this study, the Cytb and 16S gene regions of A. gossypfi collected from 5 common summer hosts and 4 primary hosts were analyzed. A total of 57 haplotypes were obtained from 1 046 individual A. gossyp# sequences. The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites. The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual. A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals. Considering the individual host plant, 5 biotypes were identified. Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races. Type 3 was the most abundant biotype in cotton fields in northern China.

关 键 词：cotton-melon aphid Cytb and 16S genes haplotype BIOTYPE cotton field 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治]