香芩解热颗粒对2,4-二硝基苯酚致热大鼠的解热作用研究  

Antipyretic Effect of Xiangqin Jiere Granule(香芩解热颗粒)on 2,4-dinitrophenol Induced Febris Rats

作　　者：聂坚[1] 李刚 张建英[1] 胡文敏 熊磊[1] NIEJian;LIGang;ZHANG Jianying;HUWenmin;XIONG Lei(Yunnan University of Chinese Medicine,Kunming,Yunnan 650200;Yueyang Hospital of Integrated Chinese and Western Medicine,Shanghai University of Chinese Medicine,Shanghai 200437)

机构地区：[1]云南中医学院,云南昆明650500 [2]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《中国中医药科技》2018年第4期477-480,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：云南省科技厅应用基础面上项目(2014FZ080);云南省教育厅科学研究基金项目(2014Y237);云南中医学院中医治未病理论应用研究省创新团队(云科人发[2016]10号)

摘　　要：目的:观察香芩解热颗粒对非感染性动物模型的解热作用并探讨其机制。方法:将进行体温筛选后的大鼠随机分为正常组、模型组、布洛芬组(阳性对照组)以及香芩解热颗粒组(XQ组)。除正常对照组外,其余各组背部皮下注射2,4-二硝基苯酚,复制大鼠非感染性发热模型,造模前预防性给药两次(间隔0.5 h),监测造模后0.5、1、2、3 h肛温,应用酶联免疫法(ELISA)测定大鼠血清IL-1β(白细胞介素-1β)、TNF-α(肿瘤坏死因子α)及下丘脑PGE_2(前列腺素E2)、c AMP(环磷酸腺苷)含量,应用PCR技术测定下丘脑PGE_2受体EP1、EP4 mRNA相对表达量。结果:模型组大鼠在造模后的0.5~3 h内体温均显著高于正常对照组(P<0.05),造模0.5 h达到峰值;与模型组比较,造模0.5 h后香芩解热颗粒组大鼠体温均显著降低(P<0.05)。ELISA结果显示:与正常对照组相比,模型组大鼠血清IL-1β、TNF-α及下丘脑PGE_2、c AMP含量显著上升;与模型组比较,布洛芬组大鼠血清IL-1β、TNF-α及下丘脑PGE_2、c AMP值显著下降,但香芩解热颗粒组仅下丘脑PGE_2、c AMP值显著下降。PCR结果显示:与正常对照组比较,模型组EP1mRNA、EP4mRNA相对表达量显著上升,与模型组比较,香芩解热颗粒组EP1mRNA、EP4mRNA显著下降,布洛芬组EP1mRNA显著下降。结论:香芩解热颗粒对酵母型发热大鼠有明显的退热趋势,其退热作用可能与降低下丘脑体温正调节介质PGE_2、c AMP的含量及下调下丘脑EP1mRNA、EP4mRNA表达有关。Objective：To investigate the antipyretic effect of Xiangqin Jiere Granule on 2,4-dinitrophenol induced febris rats.Methods：40 rats were selected and radomly divided into normal group,model group,Xiangqin Jiere Granule group and ibuprofen group.Prophylactically and respectively intragastric administrated each group with distilled water,17 g/kg Xiangqin Jiere Granule and 2.52 m L/kg ibuprofen.0.5 hours later,intragastric administrated again and established fever model by sc giving 2 mg/kg 2,4-dinitrophenol on the back,except for the normal group.The anal temperature at 0.5,1,2,3 h,the serum level of IL-1β and TNF-α,and the level of PEG2,c AMP,EP1 mRNA,EP4 mRNA in hypothalamus were all detected.Results：The body temperater of model group rats were gradually increased,and reached peak at 0.5 h.Compare with the model group,Xiangqin Jiere Granule can significantly decreased the temperature of febris rats（ P〈0.05）.The ELISA and PCR results indicated that Xiangqin Jiere Granule can significantly decreased the level of PEG2 and c AMP in hypothalamus,as well as the EP1 mRNA and EP4 mRNA expressions in hypothalamus（ P〈0.05）,but not the serum level of IL-1β and TNF-α.Conclusion：Xiangqin Jiere Granule has a good antipyretic activity to p-nitrophenol-induced febris rats.Its mechanism may be related to the inhibition of hypothalamic signal molecule（ PEG2 and c AMP） and the down-regulation of EP1 mRNA and EP4 mRNA expressions in hypothalamus.

关 键 词：香芩解热颗粒 解热作用 白细胞介素-1Β 肿瘤坏死因子-Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]