Saikosaponin a increases interleukin-10 expression and inhibits scar formation after sciatic nerve injury  

作　　者：Meng-Qiang Huang Xiao-Yu Cao Xu-Yi Chen Ying-Fu Liu Shuang-Long Zhu Zhong-Lei Sun Xian-Bin Kong Jing-Rui Huo Sai Zhang Yun-Qiang Xu 

机构地区：[1]Graduate School,Tianjin Medical University [2]Department of Orthopedics,Tianjin Medical University General Hospital [3]Department of Rehabilitation Medicine,First Affiliated Hospital of Anhui Medical University [4]Tianjin Key Laboratory of Neurotrauma Repair,Institute of Traumatic Brain Injury and Neuroscience,Center for Neurology and Neurosurgery of Affiliated Hospital,Logistics University of Chinese People's Armed Police Force [5]Science and Technology Experiment Center,Cangzhou Medical College [6]Graduate School,Jinzhou Medical University [7]Graduate School,Tianjin University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2018年第9期1650-1656,共7页中国神经再生研究（英文版）

基　　金：financially supported by the National Natural Science Foundation of China,No.11672332,11102235,8167050417;the National Key Research and Development Plan of China,No.2016YFC1101500;the Key Science and Technology Support Foundation of Tianjin City of China,No.17YFZCSY00620;the Natural Science Foundation of Tianjin City of China,No.15JCYBJC28600,17JCZDJC35400

摘　　要：Nerve scarring after peripheral nerve injury can severely hamper nerve regeneration and functional recovery.Further,the anti-inflammatory cytokine,interleukin-10,can inhibit nerve scar formation.Saikosaponin a（SSa） is a monomer molecule extracted from the Chinese medicine,Bupleurum.SSa can exert anti-inflammatory effects in spinal cord injury and traumatic brain injury.However,it has not been shown whether SSa can play a role in peripheral nerve injury.In this study,rats were randomly assigned to three groups.In the sham group,the left sciatic nerve was directly sutured after exposure.In the sciatic nerve injury（SNI） ＋ SSa and SNI groups,the left sciatic nerve was sutured and continuously injected daily with SSa（10 mg/kg） or an equivalent volume of saline for 7 days.Enzyme linked immunosorbent assay results demonstrated that at 7 days after injury,interleukin-10 level was considerably higher in the SNI ＋ SSa group than in the SNI group.Masson staining and western blot assay demonstrated that at 8 weeks after injury,type I and III collagen content was lower and nerve scar formation was visibly less in the SNI ＋ SSa group compared with the SNI group.Simultaneously,sciatic functional index and nerve conduction velocity were improved in the SNI ＋ SSa group compared with the SNI group.These results confirm that SSa can increase the expression of the anti-inflammatory factor,interleukin-10,and reduce nerve scar formation to promote functional recovery of injured sciatic nerve.Nerve scarring after peripheral nerve injury can severely hamper nerve regeneration and functional recovery.Further,the anti-inflammatory cytokine,interleukin-10,can inhibit nerve scar formation.Saikosaponin a（SSa） is a monomer molecule extracted from the Chinese medicine,Bupleurum.SSa can exert anti-inflammatory effects in spinal cord injury and traumatic brain injury.However,it has not been shown whether SSa can play a role in peripheral nerve injury.In this study,rats were randomly assigned to three groups.In the sham group,the left sciatic nerve was directly sutured after exposure.In the sciatic nerve injury（SNI） ＋ SSa and SNI groups,the left sciatic nerve was sutured and continuously injected daily with SSa（10 mg/kg） or an equivalent volume of saline for 7 days.Enzyme linked immunosorbent assay results demonstrated that at 7 days after injury,interleukin-10 level was considerably higher in the SNI ＋ SSa group than in the SNI group.Masson staining and western blot assay demonstrated that at 8 weeks after injury,type I and III collagen content was lower and nerve scar formation was visibly less in the SNI ＋ SSa group compared with the SNI group.Simultaneously,sciatic functional index and nerve conduction velocity were improved in the SNI ＋ SSa group compared with the SNI group.These results confirm that SSa can increase the expression of the anti-inflammatory factor,interleukin-10,and reduce nerve scar formation to promote functional recovery of injured sciatic nerve.

关 键 词：nerve regeneration saikosaponin a anti-inflammatory factor inflammation interleukin-l O nerve scar peripheral nerve injury sciatic nerve injury sciatic functional index nerve conduction velocity neuroelectrophysiological function neural regeneration 

分 类 号：R285.5[医药卫生—中药学]