云芝多糖组分对酒精性肝损伤小鼠的保肝活性测试  

Hepatoprotective test of polysaccharide from Coriolus versicolor on alcoholic liver injury mice

作　　者：王康乐 陆震鸣 陈露 耿燕 许泓瑜 徐国华 史劲松 许正宏 Wang Kangle;Lu Zhenming;Chen Lu;Geng Yan;Xu Hongyu;Xu Guohua;Shi Jinsong;Xu Zhenghong(School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,School ofBiotechnology,Jiangnan University,Wuxi 214122,China;Jiangsu Shenhna Pharmaceutical Co.Ltd,Huai＇an 211600,China.)

机构地区：[1]江南大学药学院,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]江苏神华药业有限公司,江苏淮安211600 [4]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食药用菌》2018年第4期235-239,共5页Edible and Medicinal Mushrooms

基　　金：国家自然科学基金(31401931)

摘　　要：通过对云芝多糖(Coriolus Versicolor Polysaccharides,PSK)分级醇沉制备了3个分子量分布不同的组分PSK-1、PSK-2和PSK-3,并采用NIAAA酒精性肝损伤小鼠模型对这3个组分的保肝活性进行了评价。结果显示:PSK-1(400 mg/kg)的保肝活性优于PSK-2和PSK-3,能够显著降低血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白固醇(LDL-C)及组织中微量丙二醛(MDA)和游离脂肪酸(NEFA)的水平,同时提升高密度脂蛋白固醇(HDL-C)的水平。The PSK-1, PSK-2 and PSK-3 were separated by step-alcohol precipitation from C. Versicolor Polysaccharides(PSK). And then evaluated the anti-alcohol liver injury activites through the NIAAA mouse model. The results showed that PSK-1(400 mg/kg) significantly reduced alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglyceride(TG), total cholesterol(TC), low-density lipoprotein sterols(LDL-C) in serum, malondialdehyde(MDA) and nonesterified fatty acid(NEFA) in tissues, while raised the level of high-density lipoprotein sterols(HDL-C). The active of other two components are lower. It was shown that PSK-1 has the best hepatoprotective activity in the three components.

关 键 词：云芝多糖 分级醇沉 肝功能 血脂 活性评价 

分 类 号：S567.31[农业科学—中草药栽培]