离子交换法分离纯化还原型谷胱甘肽的研究  

Separation and Purification of Glutathione by Ion Exchange Resin

作　　者：崔秀云[1] 邵千飞 CUI Xiu-yun1, SHAO Qian-fei2(1 College of Chemistry and Chemical Engineering, Huangshan University,Anhui Huangshan 245041; 2 Anhui Lixing Chemical Co. , Ltd. , Anhui Jixi 245300, Chin)

机构地区：[1]黄山学院化学化工学院,安徽黄山245041 [2]安徽立兴化工有限公司,安徽绩溪245300

出　　处：《广州化工》2018年第15期84-86,95,共4页GuangZhou Chemical Industry

摘　　要：还原型谷胱甘肽是一种重要的生物活性化合物,广泛应用于食品、医药和化妆品等领域,具有重要的实际应用价值和产业前景。通过5种树脂(LX-18,732,SP,HD-8,XDA-1)对谷胱甘肽进行静态吸附研究,筛选出在酸性条件下具有较高吸附量的732阳离子树脂。确定732树脂的动态吸附工艺:吸附流速为0.5 m L/min,最佳洗脱液为氯化钠溶液,且最佳洗脱浓度为2%,洗脱流速为0.8 m L/min,在最优条件下谷胱甘肽的回收率可以达到65.08%。Glutathione （GSH） is an important biological active compound. GSH is widely used in food, medicine andcosmetics, which possesses the great value and broad application prospects. Five kinds of cation-exchange resins （LX-18,732, SP, HD-8, XDA-1） were used for the separation of glutathione extraction. The optimal ion-exchange resin 732 withrelatively high capacity under acidic conditions was selected for the adsorption of GSH. The dynamic adsorption process of732 resin was determined as follow： the adsorption velocity was 0. 5 mL/ min, the eluting rate was 0. 8 mL/ min, thepurification processed with 2% NaCl. The recovery of glutathione can reach 65. 08% under the optimal condition.

关 键 词：还原型谷胱甘肽 离子交换树脂 分离纯化 

分 类 号：Q517[生物学—生物化学] O647[理学—物理化学]