白藜芦醇通过激活Caspase-3和PARP-1影响前列腺癌细胞侵袭和凋亡  

Resveratrol affects the invasion and apoptosis of prostate cancer cells by activating caspase-3 and PARP-1

作　　者：袁逾喆 张忠泉[2] 孙庆红 聂建军[4] YUAN Yu-zhe;ZHANG Zhong-quan;SUN Qing-hong;NIE Jian-jun(a.Department of Pharmacy,b.Department of Pathology,Nanyang Hospital of Traditional Chinese Medicine,Nanyang 473003,China;Pharmacy College of Henan University,Kaifeng 475000,China;Nanyang Medical College,Nan-yang 473003,China)

机构地区：[1]南阳市中医院药剂科,河南南阳473003 [2]河南大学药学院,河南开封475000 [3]南阳市中医院病理科,河南南阳473003 [4]南阳医学高等专科学校,河南南阳473003

出　　处：《实用药物与临床》2018年第7期732-736,共5页Practical Pharmacy and Clinical Remedies

基　　金：河南省医学科技攻关计划项目(201603098)

摘　　要：目的探究白藜芦醇对前列腺癌细胞侵袭和凋亡的影响及其分子机制。方法将前列腺癌细胞分为5组,命名为对照组、Z-DEVD-FMK组、Res低剂量组、Res中剂量组、Res高剂量组,采用实时荧光定量PCR(qRT-PCR)检测PC-3细胞中Caspase-3和PARP-1 mRNA表达情况,分别采用Transwell小室和流式细胞仪检测PC-3细胞侵袭和凋亡情况,蛋白免疫印迹(Western blot)检测PC-3细胞中MMP-9表达情况。结果 Res组PC-3细胞中Caspase-3和PARP-1 mRNA表达水平显著高于对照组和Z-DEVD-FMK组(P<0.05),呈剂量依赖性;Res组PC-3细胞侵袭能力显著低于对照组和Z-DEVD-FMK组(P<0.05),呈剂量依赖性;Res组PC-3细胞凋亡率显著高于对照组和Z-DEVD-FMK组(P<0.05),呈剂量依赖性;Res组PC-3细胞中MMP-9表达水平显著低于对照组和Z-DEVD-FMK组(P<0.05),呈剂量依赖性。结论白藜芦醇能够上调Caspase-3和PARP的表达,进而诱导前列腺癌细胞凋亡,为前列腺癌的临床治疗提供一定的理论依据。Objective To investigate the effect of resveratrol on invasion and apoptosis of prostate cancer cells and its molecular mechanism. Methods Prostate cancer cells were divided into five groups,named as control group,ZDEVD-FMK group,Res low-dose group,Res medium-dose group,Res high-dose group; real time quantitative PCR（qRT-PCR） was used to detect the expressions of Caspase-3 and PARP-1 mRNA in PC-3 cells. Transwell cell and flowcytometry were used to detect the invasion and apoptosis of PC-3 cells respectively,and the expression of MMP-9 in PC-3 cells was detected by Western blot. Results The expression levels of Caspase-3 and PARP-1 mRNA of PC-3 cells in Res group were significantly higher than those in control group and Z-DEVD-FMK group（P〈 0. 05）,in a dose-dependent manner; the invasive ability of PC-3 cells in Res group was significantly lower than that in control group and Z-DEVD-FMK group（P 〈0. 05）,in a dose-dependent manner; the apoptosis rate of PC-3 cells in Res group was significantly higher than that in control group and Z-DEVD-FMK group（P〈 0. 05）,in a dose-dependent manner;the expression level of Res in PC-3 group was significantly lower than that in control group and Z-DEVD-FMK group（P 〈0. 05）,in a dose-dependent manner. Conclusion Resveratrol can up-regulate the expressions of Caspase-3 and PARP,and induce apoptosis of prostate cancer cells,which provides a theoretical basis for the clinical treatment of prostate cancer.

关 键 词：白藜芦醇 前列腺癌 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 多聚腺苷酸二磷酸核糖聚合酶 

分 类 号：R285[医药卫生—中药学]