白头翁皂苷B4在Calu-3细胞单层中的渗透性研究  

Evaluation of Anemoside B4 Permeability in Calu-3 Cell Monolayer

作　　者：狄孟华 李言 李桂凤 崔京浩[2] 王永林[1] 杨世林[1] DI Menghua;LI Yan;LI Guifeng;CUI Jinghao;WANG Yonglin;YANG Shilin(Guizhou Medical University,Guiyang 550004,Guizhou China;College of Pharmacy,Soochow University,Suzhou 215123,Jiangsu,China)

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]苏州大学药学院,江苏苏州215123

出　　处：《贵州医科大学学报》2018年第7期750-753,共4页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目资助(K113231513)

摘　　要：目的:建立白头翁皂苷B4的HPLC测定方法,研究其在单层Calu-3细胞中的渗透性。方法:色谱条件为Agilent C18柱(4.6 mm×250 mm,5μm)、流动相为0.1%磷酸-乙腈(71%~29%)、流速为0.8 m L/min、检测波长205 nm、柱温25℃,考察不同浓度B4在Calu-3单层细胞中的渗透情况,以卡波姆974P为促渗剂,考察卡波姆对B4渗透性的影响。结果:B4在1.5~30.0 mg/L内与峰面积呈良好线性关系(r=0.999 3),B4在Calu-3细胞的渗透性具有浓度依赖性,卡波姆的加入提高了B4的渗透量。结论:B4的渗透以细胞旁路为主要途径,而卡波姆的加入可提高细胞旁路通透性,使药物渗透量增加。Objective： To establish a HPLC method to investigate of anemoside B4 permeability in Calu-3 cell monolayer. Methods： The samples were analyzed with a Agilent C18 column（ 4. 6 mm ×250 mm,5 μm） at 25 ℃. The mobile phase was water containing 0. 1% H3 PO4-acetonitrile（ 71% ∶29%）. The flow rate was 0. 8 mL/min. The transport of B4 in Calu-3 monolayer cells was investigated with different concentrations of B4,and Carbopol 974 P was used as a penetration enhancer to determine the effect of carbopol on B4 permeability. Results： The line-arity of B4 calibration curve was good in the range of 1. 5 - 30. 0 mg/L（ r = 0. 999 3）. The minimal quantitation limit was 1. 5 mg/L.The penetration of B4 on Calu-3 cells was concentration-dependent,and the addition of Carbopol increased the penetration of B4. Conclusions： The absorption of B4 on mucous membrane is mainly through paracellular pathway,and the Carbopol can enhance the permeation of B4.

关 键 词：高效液相色谱 白头翁皂苷B4 Calu-3细胞 卡波姆 渗透性 

分 类 号：R285.5[医药卫生—中药学] O657.63[医药卫生—中医学]