人脐带间充质干细胞分化为心肌细胞过程中CaN-NFATc信号通路表达的变化  

Changes of CaN-NFATc signaling pathway during differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes

作　　者：阮中宝[1] 张瑞 陈各才[1] 王飞[1] 朱莉[1] RUAN Zhong-bao;ZHANG Rui;CHEN Ge-cai;WANG Fei;ZHU Li(Taizhou People＇s Hospital,Taizhou 225300,Chin)

机构地区：[1]泰州市人民医院,江苏泰州225300

出　　处：《临床医学研究与实践》2018年第23期1-3,16,共4页Clinical Research and Practice

摘　　要：目的探讨钙调神经磷酸酶(CaN)-活化T细胞核因子(NFATc)信号通路在5-氮杂胞苷(5-aza)诱导人脐带间充质干细胞(hUCMSCs)分化为心肌细胞过程中的作用。方法收集足月顺产健康产妇的脐带,于无菌条件下剪碎后体外分离、培养hUCMSCs,采用10μmol/L或20μmol/L5-aza对P3代hUCMSCs诱导24h后继续培养4周。采用RT-PCR和WesternBlot检测诱导前、后CalcineurinA、GATA4和NFATc2表达。结果与诱导前和对照组比较,10μmol/L和20μmol/L浓度诱导4周hUCMSCs的CalcineurinA、GATA4和NFATc2表达均明显上调(P<0.05或P<0.01)。10μmol/L浓度的5-aza诱导效果效果明显优于20μmol/L(P<0.05或P<0.01)。对照组4周后CalcineurinA、GATA4和NFATc2表达水平较诱导前无明显变化(P>0.05)。结论 CaN-NFATc信号通路参与hUCMSCs心肌细胞分化的调控,10μmol/L的浓度是5-aza诱导hUCMSCs心肌细胞分化的最佳浓度。Objective To investigate the role of calcineurin（CaN）-nuclear factor of activated T cells（NFATc） signaling pathway in the differentiation of human umbilical cord derived mesenchymal stem cells（hUCMSCs） into cardiomyodytes induced by 5-azacytidine（5-aza）. Methods The umbilical cord of full-term health maternal were collected, hUCMSCs were isolated and cultured in vitro after sterilizing. The 10 μmol/L or 20 μmol/L 5-aza was used to induce P3 hUCMSCs for24 h and continued for 4 weeks. RT-PCR and Western Blot were used to detect the expression of Calcineurin A, GATA4 and NFATc2 before and after induction. Results After 4 weeks, the expression levels of Calcineurin A, GATA4 and NFATc2 in hUCMSCs induced by 10 μmol/L and 20 μmol/L 5-aza significantly increased than those before induction and the control group（P0.05 or P0.01）; After 4 weeks, the effect of 5-aza induction at 10 μmol/L was significantly better than that at 20 μmol/L（P 0.05 or P 0.01）. The expression levels of Calcineurin A, GATA4 and NFATc2 in the control group were not significantly different from those before induction（P 0.05）. Conclusion CaN-NFATc signaling pathway maybe participate in regulating the differentiation of hUCMSCs into cardiomyodytes, the concentration of 10 μmol/L is the optimal concentration of 5-aza induced differentiation of hUCMSCs into cardiomyodytes.

关 键 词：CaN-NFATc 人脐带间充质干细胞(hUCMSCs) 5-氮杂胞苷(5-aza) 心肌细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]