低温锻炼期光周期对笃斯越橘幼苗生理特性的影响  

Effects of Photoperiod on the Physiological Characteristics of Vaccinium uligiuosum L. Seedlings During Cold Acclimation

作　　者：乌凤章[1] WU Fengzhang(Institute of Modern Agricultural Research,Dalian University,Dalian,Liaoning 116622)

机构地区：[1]大连大学现代农业研究院,辽宁大连116622

出　　处：《北方园艺》2018年第15期45-52,共8页Northern Horticulture

基　　金：辽宁省自然科学基金资助项目(2015020785);大连市科技计划资助项目(2015B11NC084)

摘　　要：以3年生笃斯越橘苗为试材,将苗木分别置于4℃低温下短日照(10h)和长日照(14h)人工环境中,21d后测定不同处理叶片生理指标。结果表明:低温锻炼期不同光周期对超氧化物歧化酶(SOD)活性影响较小;显著降低了过氧化物酶(POD)活性;显著提高了过氧化物酶(CAT)活性。低温锻炼明显提高蔗糖、葡萄糖、果糖和可溶性蛋白质含量,这种作用主要与低温诱导有关,而受光周期影响较小。低温锻炼期不同日照处理明显提高了赤霉素(GA3)含量,其中短日照处理下提高幅度明显大于长日照;低温短日照处理下脱落酸(ABA)含量明显降低,而低温长日照处理下变化不明显;不同光周期处理对吲哚乙酸(IAA)含量无明显影响;短日照处理下玉米素核苷(ZR)含量无明显变化,而长日照处理下明显提高;低温短日照处理下ABA/GA3和ABA/IAA下降程度明显大于低温长日照。表明笃斯越橘叶片对低温锻炼环境表现出较强的适应性。由于低温短光周期对笃斯越橘ABA、GA3合成及激素平衡影响大于低温长光周期,从而对低温适应性也有一定影响。The three-year-old seedlings of Vaccinium uligiuosum L.were selected as testing materials.The seedlings were exposed to short day（10 hours）and 4 ℃ as well as long day（14 hours）and 4 ℃in controlled conditions for 21 days respectively.The physiological indexes in leave were measured after 21 days of treatment.The results showed that compared with the control,photoperiod had no significant effect on SOD activity;the POD activity was significantly decreased;the CAT activity was significantly increased.Cold acclimation significantly increased the sucrose,glucose,fructose and soluble protein content,which was related to low temperature induction,independent of photoperiod.Compared with the control,cold acclimation significantly increased GA3 content the range of increasing at short day tratment was higher than that at long day treatment;the ABA content at short day treatment was significantly decreased,but had no obvious change at long day treatment;photoperiod had no significant effect on the IAA content during cold acclimation;the ZR content did not increase significantly at short day treatment,while significantly increased at the long day treatment.The ABA/GA3 and ABA/IAA ratios was significantly less at short day treatment than that at long day.The results showed that the leaves of Vaccinium uligiuosum L.showed strong cold adaptability during cold acclimation.The effect of low temperature and short photoperiod on synthesis of ABA and GA3 and hormone balance was greater than that of low temperature and long photoperiod,therefore,it also had certain influence on the cold adaptability.

关 键 词：笃斯越橘 低温锻炼 光周期 激素 冷适应性 生理响应 

分 类 号：S666.204.3[农业科学—果树学]