不同来源、不同炮制方法的补骨脂对成骨细胞SaOS-2骨形成功能的影响  

Effects of Psoralea corylifol L. from different sources and different processing methods on bone formation of osteoblast-like SaOS-2 cells

作　　者：杨健[1,2] 陆定艳 彭潇 董莉 沈艳珍[4] 李勇军 席晓岚[1,2] 刘亭[1] YANG Jian;LU Ding-yan;PENG Xiao;DONG Li;SHEN Yan-zhen;LI Yong-jun;XI Xiao-lan;LIU Ting(Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,Guizhou,CHINA;School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,CHINA;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM（Ministry of Education）,Guizhou Medical University,Guiyang 550004,Guizhou,CHINA;Guizhou Weikang Zifan Pharmaceutical Co.,Ltd,Xiuwen 550200,Guizhou,CHINA)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [4]贵州维康子帆药业股份有限公司,贵州修文550200

出　　处：《海南医学》2018年第15期2140-2143,共4页Hainan Medical Journal

基　　金：贵州省科技计划项目(编号:黔科合J重大字[2015]2002;黔科合LH字[2016]7373);贵州省教育厅项目(编号:黔教合重大专项字[2015]040);贵州省创新人才团队项目(编号:黔科合平台人才[2016]5613;黔科合平台人才[2016]5677)

摘　　要：目的研究不同来源、不同炮制方法的补骨脂对成骨细胞SaOS-2骨形成功能的影响。方法实验分为补骨脂给药组和对照组,体外培养SaOS-2细胞,给药组分别给予不同批次、不同炮制方法的补骨脂(100 mg/L),四甲基偶氮唑盐比色法(MTT法)检测成骨细胞的增殖;PNPP法测定碱性磷酸酶(ALP)活性;酶联免疫吸附测定法(ELISA法)检测成骨细胞骨钙素(OTC)及Ca2+含量;茜素红染色法检测细胞矿化结节数。结果与对照组比较,不同来源的补骨脂均能显著促进SaOS-2细胞增殖、分化及矿化,差异均有统计学意义(P<0.05);酒制补骨脂、清炒补骨脂、雷公补骨脂、盐炙补骨脂及补骨脂生品各组的细胞增殖率依次为(118.01±0.38)%、(118.00±0.76)%、(121.01±0.88)%、(120.21±0.78)%、(117.21±0.78)%,ALP活性分别为(909.52±33.44)U/g、(915.45±23.04)U/g、(927.89±23.29)U/g、(938.52±23.94)U/g、(900.52±23.94)U/g,Ca2+的分泌分别为(182.79±5.72)mg/L、(183.74±6.01)mg/L、(185.89±7.23)mg/L、(187.79±6.01)mg/L、(180.79±6.01)mg/L,OTC的分泌分别为(15.41±0.77)μg/L、(15.42±0.56)μg/L、(15.40±0.68)μg/L、(15.58±0.68)μg/L、(15.41±0.68)μg/L,细胞矿化结节数依次为(10.52±0.77)个、(10.55±0.65)个、(10.59±0.47)个、(10.74±0.77)个、(10.50±0.77)个,以上各指标与对照组[(100.00±0.53)%、(411.62±15.46)U/g、(25.25±1.47)mg/L、(11.41±0.63)μg/L、(4.20±0.60)个]相比,差异均有统计学意义(P<0.05)。雷公补骨脂和盐炙补骨脂的细胞增殖率分别为(121.01±0.88)%、(120.21±0.78)%,明显高于补骨脂生品的(117.21±0.78)%,差异均有显著统计学意义(P<0.01);盐炙补骨脂与补骨脂生品的ALP活性[(938.52±23.94)U/g vs(900.52±23.94)U/g]相比,差异有统计学意义(P<0.05)。结论补骨脂药材的来源对其促成骨细胞骨形成的作用无明显影响;补骨脂药材经炮制后促进成骨细胞骨形成的作用较生品更优。Objective To study the effects of Psoralea corylifol L. from different sources and different processing methods on bone formation function of osteoblast-like SaOS-2 cells. Methods SaOS-2 cells were cultured in vitro and divided into P. corylifolia administration groups and control group. The cells in the administration groups were treated with P. corylifolia（100 mg/L） from different sources and different processing methods. Cell proliferation was measured by MTT assay, and the activity of alkaline phosphatase（ALP） was measured by PNPP method. Secretions of Ca2＋,osteocalcin（OTC） were detected by ELISA assay, and osteoblast mineralization nodules were measured by alizarin red staining method. Results Compared with the control group, P. corylifolia from different sources could significantly promote the proliferation, differentiation and mineralization of SaOS-2 cells（P〈0.05）. The cell proliferation rates of each group of wine fried P. corylifolia, plain-fried P. corylifolia, P. corylifolia processed by tripterygium wilfordii method,fried salt P. corylifolia and raw of P. corylifolia were（118.01 ± 0.38）%,（118.00 ± 0.76）%,（121.01 ± 0.88）%,（120.21 ±0.78）%,（117.21±0.78）%, and ALP activity were（909.52±33.44） U/g,（915.45±23.04） U/g,（927.89±23.29） U/g,（938.52±23.94） U/g,（900.52±23.94） U/g, with Ca2 ＋secretion of（182.79±5.72） mg/L,（183.74±6.01） mg/L,（185.89±7.23） mg/L,（187.79±6.01） mg/L,（180.79±6.01） mg/L, OTC secretion of（15.41±0.77） μg/L,（15.42±0.56） μg/L,（15.40±0.68） μg/L,（15.58±0.68） μg/L,（15.41±0.68） μg/L, and the number of mineralized nodules of（10.52±0.77）,（10.55±0.65）,（10.59±0.47）,（10.74 ± 0.77）,（10.50 ± 0.77）. The above indicators all showed statistically significant difference with（100.00 ±0.53）%,（411.62±15.46） U/g,（25.25±1.47） mg/L,（11.41±0.63） μg/L,（4.20±0.60） in the control group（P〈0.05）. The cell proliferation rates of P. corylif

关 键 词：补骨脂 炮制方法 不同来源 成骨细胞 骨形成 

分 类 号：R282.71[医药卫生—中药学]