植物microRNA靶基因的预测与验证技术研究进展  

Research Progress on Predication and Validation Technologies of MicroRNA Targets in Plants

作　　者：崔俊霞 魏康宁 谢伊源 王丽[1] 李用芳[1] CUI Junxia, WEI Kangning, XIE Yiyuan, WANG Li, LI Yongfang(College of Life Sciences, Henan Normal University, Xinxiang 453007, Chin)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南农业科学》2018年第7期1-7,共7页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金(31771703);河南师范大学研究生科研创新项目(YL201723);国家大学生创新训练项目(201610476027)

摘　　要：MicroRNA(miRNA)是真核生物中具有重要调控功能的一类小分子RNA。植物miRNA通过碱基互补配对方式引导RNA沉默复合体识别靶基因并介导靶基因mRNA被剪切或蛋白质翻译被抑制,所以对植物miRNA靶基因的分析和验证是研究miRNA功能必不可少的一部分。植物miRNA靶基因可通过生物信息学方法预测,主要根据miRNA与mRNA的互补程度进行,然而理论上预测的靶基因尚有待于进一步的试验验证。简要介绍了植物miRNA产生过程的最新研究进展以及靶基因的预测方法,重点综述了植物miRNA靶基因的验证方法,包括RLM-5'RACE、降解组测序、瞬时共表达和转基因系统,为更深入地研究植物miRNA介导的调控机制提供参考。MicroRNA （miRNA）is a class of small RNAs with important regulatory functions in eukaryotes. In plants, miRNA recognizes its target mRNA based on sequence complementarity, and regulates it by the mechanism of cleavage or translational repression, so analysis and validation of miRNA targets are essential for study of miRNA functions, miRNA targets can be predicted by bioinformatics based on sequence complementarity between miRNA and candidate target mRNA, while further experimental validation is required to confirm the authenticity of the predicted targets. The present review briefly summarized the recent progress on miRNA biogenesis in plant, and the pipelines used for miRNA targets prediction. The techniques used for miPtNA target validation, such as HLM-5＇RACE, degradome sequencing, transient co-expression and transgenic system, were systematically summarized in this review, which is useful for elucidating the regulation mechanism mediated by plant miRNA.

关 键 词：植物MIRNA 靶基因 剪切 预测 验证 

分 类 号：S184[农业科学—农业基础科学] Q943.2[生物学—植物学]