谷氨酰胺对施氏鲟胚胎发育及其生化指标的影响  

Effects of Glutamine on Development and Biochemical Indexes of Acipenser schrenckii Embryos

作　　者：谢先中[1] 陈晓武[2] 冉光鑫[1] 杨斌[1] 王永树[1] 

机构地区：[1]贵州省黔西南民族职业技术学院,贵州兴义562400 [2]上海海洋大学水产与生命学院,上海200090

出　　处：《贵州农业科学》2018年第7期100-102,共3页Guizhou Agricultural Sciences

基　　金：黔西南州科技局科技计划项目"孵化生态因子调控及保护性抗氧化剂添加降低ROS对鲟鱼胚胎发育氧化损伤研究"(2017-22);上海市科委基础重大项目(073205109);上海市重点学科水生生物学建设项目(S30701)

摘　　要：为提高施氏鲟胚胎发育及抗氧化损伤能力,在孵化水体中添加谷氨酰胺(Gln),以观察其对施氏鲟胚胎发育及其生化指标的影响。结果表明:添加Gln的各处理较对照的孵化率、可溶性蛋白含量、SOD酶活力和CAT酶活力分别提高3.50%~11.86%、10.29%~17.43%、8.74%~16.99%和4.10%~15.90%,畸形率和MDA含量降低幅度分别为-51.90%^-12.66%和-20.83%^-12.50%;5.0mg/L的Gln为最佳添加量。Gln能促进施氏鲟胚胎发育、提高孵化率、促进抗氧化酶合成,并可降低畸形率和MDA含量。The embryonic development and biochemical indexes of Acipenserschrenckiiembryos in hatching water containing different Gln concentration were determined to improve development and antioxidant damage ability of A.schrenckii embryos.Result：The hatching rate,soluble protein content,SOD activity and CAT activity of A.schrenckii embryos in hatching water containing different Gln concentration are 3.50%-1.86%,10.29%-17.43%,8.74%-16.99% and 4.10%-15.90% higher than CK respectively and the decrease range in deformity rate and MDA content of A.schrenckii embryos in hatching water containing different Gln concentration is-51.90%--12.66% and-20.83%--12.50%compared with CK separately.The optimum Gln concentration for embryonic development of A.schrenckii is 5.0 mg/L.Gln can promote embryonic development of A.schrenckii,improve hatching rate of A.schrenckii embryos,accelerate antioxidase synthesis and reduce embryonic deformity rate and MDA content.

关 键 词：谷氨酰胺 施氏鲟 胚胎发育 生化指标 

分 类 号：S963.731[农业科学—水产养殖]