免疫胶体金标记法研究筛管特异质体中微丝来源的细胞骨架成分  

Component analyzing of the micro-filament originated cytoskeleton protein of sieve element specific plastid by using of immuno-gold labeling

作　　者：宋西娇 廖珍凤 谢礼[1,3] 张恒木[3] 洪健 SONG Xi-jiao;LIAO Zhen-feng;XIE Li;ZHANG Heng-mu;HONG Jian(Central Laboratory of Zhejiang Academy of Agricultural Sciences,Hangzhou Zhejiang 310021;Institute of Virology and Biotechnology’Zhejiang Academy of Agricultural Sciences’Hangzhou Zhejiang 310021;Analysis Center of Agrobiology and Environmental Science’Zhejiang University’Hangzhou Zhejiang 310058’China)

机构地区：[1]浙江省农业科学院公共实验室,浙江杭州310021 [2]浙江大学分析测试中心,浙江杭州310058 [3]浙江省农业科学院病毒与生物技术研究所,浙江杭州310021

出　　处：《电子显微学报》2018年第4期376-380,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学青年基金项目(31600123)

摘　　要：叶绿体等细胞器在细胞内的位置维持和运动受细胞骨架的调控。筛管特异质体是筛管细胞内的特征性细胞器,其发生与叶绿体同源。研究利用免疫胶体金标记法结合商品化的微丝蛋白抗体,发现微丝蛋白actin和myosin除了能被标记到叶绿体上,还能被标记到筛管特异质体上。该结果暗示了微丝蛋白可能也参与了筛管特异质体在筛管细胞中的位置维持和运动,为细胞器运动的研究提供了新线索。Cytoskeleton plays an important role in controlling the movement or maintaining the location of organelle in cell,such as chloroplast. Specific sieve element（ SE） plastid is the peculiar organelle of sieve element,whose development pattern resembles to chloroplast. By using of immuno-gold labeling method combining with the commercial antibody against micro-filament protein actin and myosin,we found that actin and myosin can be labeled not only on chloroplast but also on specific SE plastid. This result implied that micro-filamentous protein may also take part in controlling the movement or maintaining the location of specific SE plastid,which provided new clues in the research of organelle movement.

关 键 词：筛管特异质体 微丝 蛋白成分 免疫胶体金标记法 

分 类 号：Q942[生物学—植物学] Q336