柚皮苷对牙本质中胶原酶活性的影响  

The effects of naringin on the activity of collagenase from dentin

作　　者：刘正雅 余昊翰 许荣宸 余凡 陈吉华 李芳 LIU Zheng-ya;YU Hao-han;XU Rong-chen;YU Fan;CHEN Ji-hua;LI Fang(State Key Laboratory of Military Stomatology ＆ National Clinical Research Center for Oral Diseases ＆Shaanxi Key Laboratory Oral Diseases,Department of Prosthodontics,School of Stomatology,The Fourth Military Medical University,Xi′an 710032,China)

机构地区：[1]军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病重点实验室第四军医大学口腔医院修复科,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2018年第7期373-377,共5页Chinese Journal of Conservative Dentistry

基　　金：国家重点研发计划重点专项课题(2017YFC0840100;2017YFC0840109);国家自然科学基金(81571019;81720108011;81470773);教育部创新团队发展计划(IRT13051);陕西省重点研发计划项目(2015SF148;2017SF-115)

摘　　要：目的:检测柚皮苷(Ng)对游离型和牙本质结合型胶原酶活性的影响。方法:用荧光定量法检测125、250、500μg/m L Ng对游离型胶原酶活性的影响。制备牙本质胶原样本24个,随机分为3组(n=8):去离子水处理组(空白组);500μg/m L Ng处理组,2 g/L氯已定处理组;各组分别处理10 min后,将牙本质胶原样本分别置于老化液中15 d,检测胶原的干重变化、羟脯氨酸释放量。另制备牙本质片6个,经100 g/L H3PO4处理60 s后,随机分为2组(n=3),实验组为500μg/m L Ng处理10 min;空白组不做任何处理,用原位明胶酶实验评估Ng对牙本质结合型胶原酶活性的影响。结果:500μg/m L Ng的酶抑制率(83.17%)高于125、250μg/L Ng;500μg/L Ng处理老化15 d后的牙本质胶原样本,其胶原干重丢失比例、羟脯氨酸释放量均显著低于空白组(P<0.05);原位明胶酶实验结果显示,500μg/m L Ng处理后的样本荧光释放量显著低于空白组(P<0.05)。结论:500μg/m L Ng对游离型和牙本质结合型胶原酶均有抑制作用。AIM： To study the effects of naringin（Ng） on the activity of soluble collagenase and dentin matrix-bound collagenase. METHODS： A fluorometric assay was used to examine the inhibitory effects of Ng at different concentrations on soluble collagenase. Matrix-bound collagenases activity was evaluated via dry mass loss and hydroxyproline （HYP） release from naringin pretreated demineralized dentin after aging for 15 days, the experiment of in situ zymography was used to investigate the effect of naringin on matrix-bound collagenases activity. RESULTS： Ng at 500 μg/mL inactivated the soluble collagenase by 83.17%. Compared with control group, pretreated demineralized dentin beams exhibited less dry mass loss, lower hydroxyproline release after 15 days aging. Dentin samples pretreated with Ng showed lower enzyme activities examined by the in situ zymography. CONCLUSION： Naringin can inhibit the activity of both soluble and dentin matrix-bound collagenases.

关 键 词：柚皮苷(Ng) 胶原酶 牙本质胶原 

分 类 号：R780.2[医药卫生—口腔医学]