穿心莲内酯抑制肿瘤细胞分泌物诱导的血管新生  

Inhibitory effect of andrographolide on angiogenesis induced by the supernatant from cultured tumor cells

作　　者：郭晓兰[1] 赵茂州 林玉茵 陈文生[1] 王诗雯 戴建威[1] GUO Xiaolan;ZHAO Maozhou;LIN Yuyin;CHEN Wensheng;WANG Shiwen;DAI Jianwei(GMU-GIBH ]oint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,China)

机构地区：[1]广州医科大学一中国科学院广州生物医药与健康研究院联合生命科学院,广州511436

出　　处：《中南大学学报（医学版）》2018年第8期821-825,共5页Journal of Central South University ：Medical Science

基　　金：广东省自然科学基金(2016A030313601);广东省科技发展专项资金(2017A020211009);广州市科技局科技计划项目(201510010076);广州医科大学基础学院学生课外学术科技项目伍(XS201618).

摘　　要：目的:探讨穿心莲内酯(andrographolide,Andro)对人脐带静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)成管能力的影响。方法:将不同体积浓度的Andro溶液作用于HUVECs,采用细胞计数试剂盒-8(Cell Counting Kit-8,CCK-8)检测细胞存活率。采用半数致死量(IC50)的Andro溶液作用于HUVECs,采用Matrigel胶使HUVECs成管,通过计算成管率来评估Andro溶液对HUVECs成管能力的影响。此外,将A549细胞分泌物与Andro溶液同时处理HUVECs,检测其成管情况。采用Western印迹检测Andro溶液对基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达水平的影响。结果:Andro溶液可抑制HUVECs的增殖,且呈浓度依赖性;Andro溶液对HUVECs的IC50为20μmol/L。选取20μmol/L的Andro溶液作用于HUVECs 24 h,发现Andro溶液显著抑制HUVECs的成管能力。另外,A549细胞分泌物促进HUVECs成管作用,而Andro溶液拮抗肿瘤细胞分泌物对HUVECs成管的促进作用,降低MMP-9的表达。结论:Andro能够抑制HUVECs成管,而且能够拮抗肿瘤细胞分泌物对HUVECs成管的促进作用,从而抑制血管新生。Objective： To determine the effect of andrographolide （Andro） on angiogenesis of human umbilical vein endothelial cells （HUVECs）. Methods： HUVECs were treated with different concentrations of Andro and the cell viability wasdetected with Cell Counting Kit-8 （CCK-8）. HUVECs were treated with half lethal dose （ICs0） ofAndro. Matrigel was used to make capillary formation of HUVECs and the effect of Andro on capillary formation was evaluated by calculating the percentage of capillary formation. Moreover, the effects of Andro and the supernatant from cultured A549 tumor cells on capillary formation were evaluated by calculating the percentage of capillary formation. The effect of Andro on the expression of matrix metaUoproteinase-9 （MMP-9） was determined with Western blot. Results： ]-he cell viability of HUVECs decreased with the increase of Andro concentrations. ICs0 was 20 [~mol/L. ]-he capillary formation of HUVECs was inhibited when treated with 20 ~tmol/L Andro for 24 hours. Moreover, Andro was able to antagonize the promotion of the capillary formation induced by the supernatant from cultured tumor cells. Andro could suppress the expression of MMP-9 and antagonize the capillary formation. Conclusion： Andro inhibits the capillary formation of HUVECs and can antagonize the promotion of angiogenesis induced by the supernatant from cultured tumor cells.

关 键 词：穿心莲内酯 人脐带静脉内皮细胞 细胞活力 肿瘤细胞 血管新生 

分 类 号：R285[医药卫生—中药学]