饲料脂肪水平对血鹦鹉幼鱼肝脏免疫及抗氧化酶的影响  

Effects of dietary lipid levels on immune enzymes and hepatic antioxidation of blood parrot juvenile

作　　者：薛晓强 赵月 王帅 赵琳 胥亚夫 王友权 王睿瑞 任同军 韩雨哲 XUEXiaoqiang;ZHAOYue;WANGShuai;ZHAOLin;XUYafu;WANGYouquan;WANGRuirai;RENTongjun;HANYuzhe(College of Fisheries and Life Scuence,Dalian Qcean University Key Laboratory of MariculChina’s Sea,Ministry of Agriculture Key Laboratory of Fish Applied Biology and Aquaculture in Northern China,Liaoning Province,Dalian 116023,China;Liaoning Fishery Antarctic Krill Technology DevelopmentCo.L td.,Dalian 116113,China)

机构地区：[1]大连海洋大学水产与生命学院农业部北方海水增养殖重点实验室辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023 [2]辽渔南极磷虾科技发展有限公司,辽宁大连116113

出　　处：《中国渔业质量与标准》2018年第3期61-67,共7页Chinese Fishery Quality and Standards

基　　金：大连海洋大学博士启动项目;大连海洋大学大学生创新创业训练计划项目(201610158000039);辽宁省博士启动基金(201601289)

摘　　要：为研究饲料脂肪水平对血鹦鹉幼鱼(Cichlasoma synspilum♀×Cichlasoma citrinellum♂)肝脏免疫及抗氧化酶的影响,以豆油为脂肪源,配制5组粗脂肪含量分别为4.77%(CL1)、8.75%(CL2)、11.68%(CL3)、16.25%(CL4)、23.70%(CL5)的实验饲料饲喂血鹦鹉幼鱼。实验采用初始体重为(1.20±0.30)g的血鹦鹉幼鱼分5个不同脂肪饲料来饲养42 d,每组30尾。饲养6周后,无菌取其肝脏,制备组织匀桨液,测定其肝脏中免疫及抗氧化相关酶含量,包括碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、溶菌酶(LMZ)、超氧化物歧化酶(SOD)、总抗氧化酶(T-AOC)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)。实验结果表明,饲喂不同饲料脂肪水平的血鹦鹉幼鱼肝脏免疫及抗氧化相关酶活力均呈现先升高后下降的趋势;在饲料脂肪水平为11.68%时,LMZ、SOD、T-AOC、GSHPx活力最高且显著高于其他脂肪水平组(P<0.05);当脂肪水平为16.25%时,AKP、ACP、CAT活力、MDA达到最大值并显著高于其他饲料组(P<0.05)。通过绘制二次曲线回归方程得知血鹦鹉幼鱼最适饲料脂肪水平为15.80%~16.75%。本研究将为血鹦鹉幼鱼实用饲料营养的精准供给提供理论基础。The current study was to investigate effects of various dietary lilpid levels on the immune enzymes and hepatic antioxidant activity of Cichlasoma synspilum ♀ × Cichlasom citrinellum ♂. 5 various treatments of dietary lipid contents（ 4. 77%,CL1; 8. 75%,CL2; 11. 68%,CL3; 16. 25%,CL4 and 23. 70%,CL5） using soybean oil as the source of fat were performed. The blood parrot juveniles with starting weight of（ 1. 20 ± 0. 30） g were randomly divided into 5 groups（ 30 of each group） and feeded for 42 days. After feeding dietary lipid,hepatic immune enzymes（ alkaline phosphatase,AKP; acid phosphatase,ACP; lysozyme,LMZ） and antioxidant activities（ total antioxidant enzyme,T-AOC; superoxide dismutase,SOD; catalase,CAT; glutathione peroxidase,GSH-Px; malondialdehyde,MDA） were determined. It was observed that they were increased first and then decreased. Higher LMZ,SOD,TAOC and GSH-Px activities were found when juveniles were feeded with dietary lipid of 11. 68% compared with those of other groups. Additionally,they showed a significantly higher content（ P 0. 05）. The activities of liver AKP,ACP,CAT,and MDA of the blood parrot juveniles reached their maximum contents when fed with 16. 25% dietary lipid and were also significantly higher than those of other groups（ P 0. 05）. To explore the recommended content for blood parrot juveniles,regression equation was developed and the optimum dietary lipid level for blood parrot juveniles is 15. 80%-16. 75%. These data may be benefit for the blood parrot juvenile to develop a practical feed.

关 键 词：血鹦鹉 饲料脂肪水平 幼鱼 抗氧化酶 免疫酶 二次曲线回归 

分 类 号：S963.1[农业科学—水产养殖]