Availability of Myoglobin as a Molecular Marker for Phylogenetic Relationships of Fish  

作　　者：Ming-Chih Huang Yoshihiro Ochiai Shugo Watabe 

机构地区：[1]Department of Biological Sciences and Technology, National University of Tainan, 33, Sec.2, Shu-Lin St.,Tainan 700-05, Taiwan R.O.C. [2]Graduate School of Agricultural Science, Tohoku University, Aramaki, Aoba-ku, Sendai, Miyagi 980-0845, Japan [3]School of Marine Biosciences, Kitasato University, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan

出　　处：《Journal of Agricultural Science and Technology(B)》2018年第2期131-143,共13页农业科学与技术（B）

摘　　要：In order to elucidate the phylogenetic relationship of fish, DNA and deduced amino acid sequences of myoglobin （Mb） were used for the phylogenetic analyses based on different approaches, namely, maximum likelihood （ML）, neighbor joining （NJ）, unweighted pair group method with arithmetic means （UPGMA） and maximum parsimony （MP） methods in comparison with the conventional molecular markers, mitochondrial cytochrome b （cyt-b） and cytochrome c oxidase subunit I （COI）. The phylogenetic trees drawn based on Mb sequences were similar to those by the traditional classification based on the other molecular markers. The primary and secondary structures, as well as the modeled tertiary structures of Mbs were similar to each other, but were clearly distinguishable among those species. Such differences in structure would be associated with adaptation of Mb molecule to the physiological conditions of each species. These results suggest that Mb can be a molecular marker for the phylogenetic relationship of fish.

关 键 词：MYOGLOBIN mitochondrial cytochrome b （cyt-b） cytochrome c oxidase subunit I （COI） phylogeny. 

分 类 号：Q51[生物学—生物化学] S852.655[农业科学—基础兽医学]