钴-60射线辐照对白术药材中4种有效成分的影响研究  

Study on the Effects of Co-60 Irradiation on 4 Effective Components in Atractylodis Macrocephalae Rhizoma

作　　者：王春雷[1] 姜建伟[1] 侯桂兰[1] Wang Chunlei;Jiang Jianwei;Hou Guilan(Zhejiang Cancer Hospital,Hangzhou 310022,China)

机构地区：[1]浙江省肿瘤医院,杭州310022

出　　处：《中国药师》2018年第8期1358-1362,共5页China Pharmacist

摘　　要：目的:研究不同剂量钴-60(60Co)射线辐照对白术药材4种有效成分含量的影响。方法:采用HPLC-DAD梯度洗脱-波长切换法;以Agilent C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为乙腈-水,梯度洗脱,流速为1.0 ml·min-1,柱温35℃,检测波长为220 nm(白术内酯Ⅱ、白术内酯Ⅲ及苍术酮)和275 nm(白术内酯Ⅰ),进样量为10μl。分别选择剂量为5,8,10 k Gy对白术药材进行辐照,比较辐照前后4种有效成分含量的变化。结果:白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮分别在0.011~0.217μg,0.011~0.212μg,0.012~0.243μg,0.094~1.885μg范围内有良好线性,平均加样回收率分别为101.3%,100.6%,100.7%和100.5%。经过5,8,10 k Gy辐照后,白术药材所含的白术内酯Ⅰ、Ⅱ、Ⅲ及苍术酮均有变化。超过5 k Gy后,苍术酮辐照前后含量变化差异有统计学意义(P<0.05)。结论:所建立的白术有效成分测定方法回收率高,重复性好,简单实用,可用于白术的质量控制。在60Co辐照剂量不超过5k Gy时,各成分变化不显著,可为白术药材辐照灭菌手段提供参考。Objective： To investigate the effects of different doses of Co-60 irradiation on four kinds of effective components in atractylodis macrocephalae rhizoma. Methods： An HPLC-DAD gradient elution-wavelength switching method was adopted. An Agilent C18 column（ 250 mm × 4. 6 mm,5 μm） was used and the mobile phase was acetonitrile-water with gradient elution at the flow rate of1. 0 ml·min-1. The detection wavelength was 220 nm for atractylodesⅡ,atractylodes Ⅲ and atractylone,and 275 nm for atractylodesⅠ,and the column temperature was 35℃. Different doses of 5,8 and 10 k Gy were selected to compare the content changes of the four active components before and after the irradiation,and the difference was investigated by paired t-test. Results： The linear range of atractylodesⅠ,atractylodes Ⅱ,atractylodes Ⅲ and atractylone was 0. 011-0. 217 μg,0. 011-0. 212 μg,0. 012-0. 243 μg and 0. 094-1. 885 μg,respectively,and the average recovery was 101. 3%,100. 6%,100. 7% and 100. 5%,respectively. The contents of atractylodesⅠ,atractylodesⅡ,atractylodes Ⅲ and atractylone were all changed after the irradiation. When the dose of irradiation was more than 5 k Gy,the difference in the content change of atractylone before and after the irradiation had statistical significance（ P〈0. 05）. Conclusion： The method is with high recovery,good repeatability and simple practicality,which can be used for the quality control of atractylodis macrocephalae rhizoma. When the irradiation dose is not more than 5 k Gy,no significant changes are found in the contents of the components,which provide reference for the irradiation sterilization for atractylodis macrocephalae rhizoma.

关 键 词：白术 钴-60射线 辐照灭菌 白术内酯Ⅰ 白术内酯Ⅱ 白术内酯Ⅲ 苍术酮 

分 类 号：R927.2[医药卫生—药学]