一测多评法同时测定三七伤药片中6种活性成分  

Determination of Six Active Constituents in Sanqi Shangyao Tablets by Quantitative Analysis of Multi-components by Single Marker

作　　者：毕晓伟[1] 张璐璐[1] 张艳[1] Bi Xiaowei;Zhang Lulu;Zhang Yan(Qingdao Municipal Hospital,Shandong Qingdao 266011,China)

机构地区：[1]青岛市市立医院,山东青岛266011

出　　处：《中国药师》2018年第8期1490-1493,共4页China Pharmacist

摘　　要：目的:建立一测多评法(QAMS)测定三七伤药片中6种活性成分含量。方法:采用HPLC法,以Waters Sunfire ODS C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为乙腈-水,梯度洗脱,流速为1.0 ml·min-1,检测波长为可变波长(0 min,230 nm;10 min,283 nm;25 min,203 nm),柱温:30℃,进样量:10μl。以柚皮苷为参照物,应用QAMS测定芍药苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1和人参皂苷Re的相对较正因子并计算其含量,同时用外标法测定以上6种有效成分的含量,并将两种结果进行分析比校。结果:一测多评法测得的芍药苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1和人参皂苷Re 5种成分计算值与外标法测定值差异无统计学意义(P>0.05)。结论:QAMS法可用于三七伤药片6种指标性成分的含量测定,且方法简单、有效、结果准确。Objective： To develop a method of quantitative analysis of multi-components by single marker（ QAMS） for six active constituents in Sanqi Shangyao tablets. Methods： An HPLC method was used,the column was Waters Sunfire ODS C18（ 250 mm ×4. 6 mm,5 μm） with the temperature of 30℃,the mobile phase was acetonitrile-water with gradient elution,the detection wavelength was changeable（ 0 min： 230 nm; 10 min： 283 nm; 25 min： 203 nm）,and the injection volume was 10 μl. Using naringin as a reference,the relative correction factor for paeoniflorin,ginsenosides Rg1,Rb1 and Re,and notoginsenoside R1 were detected by QAMS and their contents were calculated. At the same time,the contents of the above 6 effective components were measured by an external standard method,and the two results were analyzed and compared. Results： There were no statistically significant differences between the calculated values of paeoniflorin,ginsenoside Rg1,Rb1 and Re,and notoginsenoside R1 and those measured by the external standard method（ P〉0. 05）. Conclusion： ： The QAMS method can be used for the determination of six index components in Sanqi Shangyao tablets,and the method is simple,effective and accurate.

关 键 词：三七伤药片 柚皮苷 芍药苷 人参皂苷Rg_1 人参皂苷Rb_1 三七皂苷R_1 人参皂苷RE 一测多评法 

分 类 号：R927.2[医药卫生—药学]