精液中白细胞异常增高对精液质量的影响及其与男性生育力的相关性  

The Influence of the Abnormal Increase of Sperm Leukocytes on the Sperm Quality and Its Correlation with Male Fertility

作　　者：蒋敏[1] 岳焕勋[1] 李福平[1] 蒋小辉[1] 李定明[1] 余林[1] 吴应碧[1] Jiang Min;Yue Huanxun;Li Fuping;Jiang Xiaohui;Li Dingming;Yu Lin;Wu Yingbi(Human Sperm Bank ＆ Key Laboratory of Birth Defects and Related Diseases of Women and Children,West China Second Hospital of Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西第二医院人类精子库.出生缺陷与相关妇儿疾病教育部重点实验室,成都610041

出　　处：《成都医学院学报》2018年第3期291-294,共4页Journal of Chengdu Medical College

基　　金：四川省科技厅重点研发项目(No:2018FZ0043)

摘　　要：目的探讨精液中白细胞异常增高对男性精液质量各主要参数及精子运动能力的影响。方法选取2016年1月至2017年12月于四川大学华西第二医院男科门诊就诊的男性不育患者8 714例为研究对象,采用白细胞过氧化物酶染色法检测受试者精液中的白细胞,按照精液中白细胞浓度的不同将患者分为两组:非炎症组(n=7 106),白细胞浓度<1×106/mL;炎症组(n=1 608),白细胞浓度≥1×106/mL。对两组患者精液质量分析中主要检测参数及精子运动参数进行分析和比较。结果炎症组患者精液各指标的中位数(第5,95百分位数)值:液化时间为30(30,30)min,pH值为7.4(7.3,7.6),与非炎症组比较,差异无统计学意义(P>0.05);炎症组患者精液体积为2.8(0.9,5.6)mL、精子浓度为69(0.5,221)×106/mL、精子总数为188(1,694)×106/mL、精子存活率为62(37,78)%、前向运动精子百分率为45(13,60)%、正常形态精子百分比为3(1.5,5.4)%,均低于非炎症组患者的精液检测结果,差异均具有统计学意义(P<0.05)。精子各运动参数中,除侧向摆动速度和平均鞭打频率外,炎症组患者精子的曲线运动速度(VCL)、直线运动速度(VSL)、运动的直线性(LIN)、前向性(STR)和平均路径速度(VAP)均下降,与非炎症组患者比较,差异有统计学意义(P<0.05)。结论精液中白细胞异常增高将导致精子运动能力降低及精液质量下降,从而影响男性的生育能力。Objective To study the influence of the abnormal increase of sperm leukocytes on the main parameters of the sperm quality and mobility. Methods A total of 8,714 male patients with infertility treated in West China Second Hospital of Sichuan University from January of 2016 to December of 2017 were selected as the subjects and divided into the non-inflammatory group （ n =7,106） with the leukocyte concentration less than 1×10^6/mL and the inflammatory group （ n =1,608） with the leukocyte concentration no less than 1×10^6/mL according to the leukocyte concentration detected by the leukocyte peroxidase staining. The main parameters of the sperm quality and motility were analyzed and compared between the two groups. Results The medians （5th and 95th percentiles） of the sperm quality parameters in the inflammatory group were 30 （30, 30）min in the liquefying time and 7.4 （7.3, 7.6） in the pH value, and they were not statistically different from those in the non-inflammatory group （ P 〉0.05）. The medians of the sperm volume were 2.8 （0.9, 5.6） mL, those of the sperm concentration was 69 （0.5, 221）×10 6/mL, those of the total sperm count was 188 （1, 694） ×10 6/ml, those of the sperm survival rate was 62% （37%, 78%）, those of the forward motile sperm percentage was 45% （13%, 60%）, and those of the normal morphological sperm percentage was 3% （1.5%, 5.4%） in the inflammatory group, and they were significantly lower than those in the non-inflammatory group （ P 〉0.05）. The inflammatory group was also significantly different from the non-inflammatory group in all the parameters of the sperm mobility including the sperm velocity of curve line （VCL）, velocity of straight line （VSL）, linearity （LIN）, STR, and velocity of average path （VAP） except the lateral swinging speed and average whiplash frequency （ P 〉0.05）. Conclusion The abnormal increase of sperm leukocytes may lead to the decrease of the sperm motility and quality and affect the male f

关 键 词：白细胞 精液质量 精子运动能力 男性生育 

分 类 号：R698.2[医药卫生—泌尿科学]