环介导等温扩增微流控检测方法研究进展  

Progress on Studies of Loop-mediated Isothermal Amplification Detection Assay Integrated on Microfluidic Chips

作　　者：殷宏[1] 汤小玉 薛强[1] 邹明强[1] YIN Hong;TANG Xiaoyu;XUE Qiang;ZOU Mingqiang(Institute of Equipment and Technology,Chinese Academy of Inspection and Quarantine（CAIQ）,Beijing,100123,China)

机构地区：[1]中国检验检疫科学研究院装备技术研究所,北京100123

出　　处：《检验检疫学刊》2018年第3期50-53,共4页Journal of Inspection and Quarantine

基　　金：公益性行业(农业)科研专项经费(201303045);国家重大科学仪器设备开发专项(2012YQ09019701);中国检验检疫科学研究院基本科研业务费(2017JK048)

摘　　要：环介导等温扩增技术是由日本荣研株式会社的Notomi等人提出的新型核酸扩增方法,在等温条件(60~65℃)下可高效、快速、特异性地扩增靶序列。微流控分析是微全分析系统的核心关键技术,将具有微小可控、功能集成的微流控芯片分析技术与环介导等温核酸扩增技术相结合,发展快速、灵敏、特异和适合即时检测的生物分子分析技术,可为我国食品安全、重大传染病防控、肿瘤及遗传性疾病等快速检测及诊断提供强有力的技术保障。Loop-mediated isothermal amplification,is a novel nucleic acid amplification method proposed by Notomi et al.（EIKEN CHEMICAL CO.,LTD.,Japan）. Target sequences can be amplified efficiently,rapidly and specifically at the isothermal condition（60-65℃）. Microfluidic analysis is the key technology of the miniaturized total analysis system. Integration of microfluidic chips with loop-mediated isothermal nucleic acid amplification technology would improve the analysis platform for rapid,sensitive,specific and point-of-care biomolecular detection,which might provide strong technical supports for food safety,prevention and control of major infectious diseases,quick and accurate diagnosis of tumor and hereditary diseases in our country.

关 键 词：环介导等温扩增 微流控芯片 快速检测 

分 类 号：Q337[生物学—遗传学]