Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses  

作　　者：Cecilia B. Percuoco Lucas G. Gimenez Liliana N. Talavera Stefani Jorge V. Crisci Carina F. Argüelles 

机构地区：[1]Laboratorio GIGA, Instituto de Biologia Subtropical, Nodo Posadas, Universidad Nacional de Misiones (UNaM), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Jujuy 1745, N3300NFK Posadas, Misiones, Argentina [2]Catedra de Genetica Molecular, Facultad de Ciencias Exactas Quimicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Felix de Azara 1552, N3300LQH Posadas, Misiones, Argentina [3]Laboratorio de Sistematica y Biologia Evolutiva (LASBE) Facultad de Ciencias Naturales y Museo (FCNyM), Universidad Nacional de La Plata-(UNLP), Avenida 122 y 60, 1900 La Plata, Buenos Aires, Argentina

出　　处：《Journal of Forestry Research》2018年第4期978-985,共8页林业研究（英文版）

基　　金：partially supported by Consejo de Investigación y Desarrollo Tecnológico(CIDET Project 16Q409 to C.F.A.);Fondo para la Investigación Científica y Tecnológica Project(PICTO UNaM 2011 No.122 to C.F.A)

摘　　要：The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rp132-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rp132-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.

关 键 词：CPDNA cpSSRs Noncoding regions 

分 类 号：Q943.2[生物学—植物学]