DNA polymorphisms and genetic relationship among populations of Acacia leucophloea using RAPD markers  

作　　者：V. N. Mutharaian R. Kamalakannan A. Mayavel S. Makesh S. H. Kwon K.-S. Kang 

机构地区：[1]Department of Botany, Bharathiar University, Coimbatore 641 046, India [2]Institute of Forest Genetics and Tree Breeding, Coimbatore 641 002, India [3]Horticultural College and Research Institute, Tamilnadu Agricultural University, Coimbatore 641 003, India [4]Department of Forest Sciences, Seoul National University, Seoul 08826, Republic of Korea [5]ENVIS Centre on Forest Genetic Resources & Tree Improvement, IFGTB, Coimbatore 641 002, India [6]ITC R&D Centre, Peenya Industrial Area I Phase, Bangalore 560 058, India [7]Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

出　　处：《Journal of Forestry Research》2018年第4期1006-1013,共8页林业研究（英文版）

基　　金：supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET)through the AgriBioindustry Technology Development Program;funded by the Ministry of Agriculture,Food and Rural Affairs(MAFRA)(No.314009-3)

摘　　要：RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.

关 键 词：Acacia leucophloea PROVENANCES Acaciasp Genetic relatedness RAPD markers 

分 类 号：S792.99[农业科学—林木遗传育种]