促红素及其氨甲酰化衍生物对孕鼠血液流变学的影响  

Effects of erythropoietin and carbamylated erythropoietin on hemorheology of pregnant rats

作　　者：马玉姗 刁敏 林雪梅 屈艺 李浩[3] 柳慧 MA Yushan;DIAO Min;LIN Xuemei;QU Yi;LI Hao;LIU Hui(Department of Anesthesiology,West China Second University Hospital,Sichuan University,Cheng du 610041,China;Key Laboratory of Birth Defects and Related Diseases of Women and Children,Sichuan University,Ministry of Education,Chengdu 610041,China;Department of Anesthesiology,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西第二医院麻醉科,四川成都610041 [2]四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室,四川成都610041 [3]四川大学华西医院麻醉科,四川成都610041

出　　处：《西部医学》2018年第8期1111-1115,共5页Medical Journal of West China

基　　金：四川省科技厅应用基础计划项目(2014JY0184;2016SZ0048)

摘　　要：目的经孕鼠腹腔给予大剂量促红细胞生成素(EPO)及氨甲酰化促红细胞生成素(CEPO),观察EPO及CEPO对孕鼠血液流变学的影响。方法将15只孕17天SD大鼠随机分为3组:生理盐水对照组(NS组)、EPO处理组(EPO组)及CEPO处理组(CEPO组),每组5只,分别经腹腔注射0.9%NS(1mL/次)、EPO(5 000U/Kg)及CEPO(50μg/Kg),连续3天。第4天经下腔静脉采血,检测孕鼠的红系相关指标(RET#、RET%、HFR、IRF、RBC及HGB)及血液流变学指标(低切变率1全血粘度、低切变率5全血粘度、中切变率50全血粘滞度、高切变率200全血粘度、EAI及HCT)。结果 EPO组红系相关指标(RET#、RET%、HFR、IRF、RBC、HGB)均高于NS组和CEPO组,差异有统计学意义(P<0.05);EPO组血液流变学相关指标中低切变率1全血粘度、低切变率5全血粘度及HCT均高于NS组和CEPO组,差异有统计学意义(P<0.05)。结论长期大剂量给予EPO能导致孕鼠骨髓造血功能增加及血液粘滞度增加,而CEPO无该作用,说明CEPO较EPO更适合于产科应用。Objective To compare EPO and CEPO＇s influence on hemorheology of pregnant rats.Method Fifteen 17-day pregnant rats were randomly divide into NS Group（n=5）,EPO Group（n=5）and CEPO Group（n=5）.Pregnant rats were injected 0.9% NS（1 mL once a day i.p.）,EPO（5,000 U/Kg once a day i.p.）and CEPO（50μg/Kg once a day i.p.）respectively for three consecutive days.The related parameters of blood routine and hemorheology of pregnant rats were detected at4 th day.And parameters were evaluated using Pearson＇s correlation coefficient.Results RET#,RET%,HFR,IRF,RBC and HGB in EPO Group were higher than those in NS Group and CEPO Group（P〈0.05）.Blood viscosity of shear rate 1,shear rate 5 and HCT in EPO Group were higher than those in NS Group and CEPO Group（P〈0.05）.Blood viscosity of shear rate1 and 5 were positively correlated with erythrocyte aggregation index.HCT is positively related to RBC,HGB and RET#.Conclusions EPO is able to induce hematopoiesis bioactivity of bone marrow,cause an increase in HGB,blood viscosity of low shear rate and HCT,and cause changes in hemorheology in pregnant rats.However,CEPO does not have these functions.It is revealed that CEPO is more suitable for obstetric applications than EPO.

关 键 词：促红细胞生成素 氨甲酰化促红细胞生成素 血液流变学 

分 类 号：R446.1[医药卫生—诊断学] R965[医药卫生—临床医学]