Preparation of vapreotide-templated silver nanocages and their photothermal therapy efficacy  

作　　者：Ruiyan Zhu Yanji Li Kexin Bian Zhengrong Gao Dawei Gao 

机构地区：[1]Applied Chemistry Key Lab of Hebei Province,Department of Bioengineering,Yanshan University,Qinhuangdao 066004,China [2]Hebei Province Asparagus Industry Technology Research Institute,Qinhuangdao 066004,China [3]Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China

出　　处：《Chinese Journal of Chemical Engineering》2018年第7期1586-1590,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(21476190);Hebei Province Key Basic Research Fund(15961301D)

摘　　要：Vapreotide acetate （Vap） was used as a biotemplate to synthesize silver nanocages through direct co-incubation of a AgNO3 solution, following by reduction using fresh NaBH4. The characterized vapreotide-templated silver nanocages （Vap-AgNCs） presented a wide and red shifted absorption band with a maximum between 480 nm and 800 nm and possessed a uniform structure with a face-centered cubic crystal structure. The biocompatibiliW of Vap-AgNCs was assessed using the MTT method, indicating Vap-AgNCs had better biocompatibility when its concentration was lower than 2,5 × 10-4 mmol. L- 1. The photothermal characteristics of Vap-AgNCs were analyzed with laser irradiation （808 nm, 1,5 W, cm-2） and the results showed that the temperature of the Vap- AgNCs solution reached 45 ℃ starting from 25 ℃ within 5 min. Additionally, Vap-AgNCs with a laser led to HeLa cell death. Therefore, the prepared Vap-AgNCs is expected to be an effective photothermal therapy agent.Vapreotide acetate(Vap) was used as a biotemplate to synthesize silver nanocages through direct co-incubation of a AgNO_3 solution, following by reduction using fresh NaBH_4. The characterized vapreotide-templated silver nanocages(Vap-Ag ) presented a wide and red shifted absorption band with a maximum between 480 nm and 800 nm and possessed a uniform structure with a face-centered cubic crystal structure. The biocompatibility of Vap-AgN Cs was assessed using the MTT method, indicating Vap-AgN Cs had better biocompatibility when its concentration was lower than 2.5 × 10^(-4) mmol·L^(-1). The photothermal characteristics of Vap-Ag NCs were analyzed with laser irradiation(808 nm, 1.5 W·cm^(-2)) and the results showed that the temperature of the VapAg NCs solution reached 45 °C starting from 25 °C within 5 min. Additionally, Vap-Ag NCs with a laser led to HeL a cell death. Therefore, the prepared Vap-AgN Cs is expected to be an effective photothermal therapy agent.

关 键 词：Silver nanocages Vapreotide acetate BIOTEMPLATE Photothermal therapy Nanoparticles 

分 类 号：O614.122[理学—无机化学] TS974.1[理学—化学]