脉冲电磁场对卵巢切除大鼠骨组织活化T细胞核因子2和空泡型V-ATP酶mRNA表达的影响  

Effects of pulsed electromagnetic fields on the expression of NFAT2 and V-ATP mRNA in the bone of ovariectomized rats

作　　者：黄煦格 何剑全[1] 陈健[1] 王潇 黄慧[1] HUANG Xuge,HE Jianquan,CHEN Jian(Department of Rehabilitation, Zhongshan Hospital Xiamen University, Xiamen Fujian, 361004, ER.Chin)

机构地区：[1]厦门大学附属中山医院康复医学科,厦门361004 [2]广州中医药大学第一临床医学院

出　　处：《中国康复医学杂志》2018年第8期896-900,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金(81272168);福建省自然科学基金(2016J01623;2017J01375);福建省卫生计生委青年科研课题(2015-2-45)

摘　　要：目的:探讨脉冲电磁场(pulsed electromagnetic fileds,PEMF)对卵巢切除(ovariectomized,OVX)大鼠骨组织细胞核因子κB受体活化因子(receptor activator of NF-κB,RANK),活化T细胞核因子2(nuclear factor of activated T2,NFAT2)和空泡型V-ATP酶(vavcuolar H+-ATPase,V-ATP)表达的影响。方法:将48只SD大鼠随机分为假手术组(SHAM),卵巢切除组(OVX),卵巢切除+脉冲电磁场治疗组(OVX+PEMF)。OVX+PEMF组大鼠在频率8Hz,磁场强度3.8m T的PEMF下每天干预40min,干预8周和16周后检测大鼠骨密度和骨组织RANK,NFAT2和V-ATP的mRNA表达水平。结果:OVX组BMD在第8周(P<0.05)和第16周(P<0.001)均低于SHAM组。SHAM组与OVX组比较,8周时RANK表达P>0.05,而SHAM组NFAT2与V-ATP表达均低于OVX组(P<0.05,P<0.01);16周时RANK、NFAT2、VATP在OVX组表达升高(均P<0.01)。SHAM组与OVX+PEMF组比较,8周时两组RANK、NFAT2、V-ATP表达P>0.05;16周时OVX+PEMF组RANK和NFAT2表达均高于SHAM组(均P<0.01),而两组V-ATP表达P>0.05。OVX组与OVX+PEMF组相比,8周时两组RANK和V-ATP表达均P>0.05,OVX+PEMF组NFAT2表达低于OVX组(P<0.05);第16周时两组RANK表达P>0.05,NFAT2和V-ATP在OVX+PEMF组的表达均下降(P<0.01,P<0.05)。结论:PEMF可通过下调OVX大鼠骨组织中NFAT2和V-ATPmRNA的表达从而抑制破骨细胞的骨吸收,进而延缓OVX大鼠的骨丢失。Objective： To investigate the effect of Pulsed electromagnetic fields（PEMF） on the mRNA expression of Receptor activator of NF-κB（RANK）, Nuclear factor of activated T2（NFAT2） and Vacuolar H＋-ATPase（V-ATP）in the bone of ovariectomized（OVX） rats.Method： Forty eight Sprague-Dawley rats were randomly divided into SHAM group, OVX group and OVX＋PEMF group. The rats in OVX ＋ PEMF group were treated in 8 Hz and 3.6 mT PEMF for 40 minutes once a day. The level of Bone mineral density（BMD） was detected after intervention. Moreover, the mRNA expression of RANK, NFAT2 and V-ATP in bone were tested in every group at 8th and 16th weeks respectively.Result： The BMD of OVX group was lower than that of SHAM group at the 8 th week（P〈0.05） and the 16th week（P〈0.001）. Comparison between SHAM group and OVX group at the 8th week, there was no significant difference in the expression of RANK mRNA（P〉0.05）, while the expression of NFAT2 and V-ATP were increased in OVX group（P〈0.05, P〈0.01）. Furthermore, the expressions of RANK, NFAT2 and V-ATP in OVX group were higher than those in SHAM group at the 16th week（all P〈0.01）. The mRNA expressions of RANK, NFAT2 and V-ATP had no significant changes between SHAM group and OVX ＋ PEMF group at the 8th week（P〉0.05）. At the 16th week, the expressions of RANK and NFAT2 in OVX＋PEMF group were higher than those in SHAM group（all P〈0.01）, but no significant difference for the expression of V-ATP（P〉0.05）. Compared to OVX group and OVX＋PEMF group, the mRNA expressions of RANK and V-ATP at 8th weeks had no difference（P〉0.05）. However, the expression of NFAT2 was down-regulated in OVX ＋ PEMF group（P〉0.05）. The RANK expression of the two groups had no change at the 16th week（P〉0.05）, while the expressions of NFAT2 and V-ATP were decreased in OVX＋PEMF group（P〈0.01, P〈0.05）.Conclusion： PEMF can reduce the bone resorption of osteoclasts by down-regulating the mRNA expressions of NFAT2 a

关 键 词：脉冲电磁场 细胞核因子κB受体活化因子 活化T细胞核因子2 空泡型V-ATP 

分 类 号：R681[医药卫生—骨科学] R493[医药卫生—外科学]