宁夏汉族与回族老年女性原发性骨质疏松症降钙素受体基因多态性研究  

Research on gene polymorphism of calcitonin receptor among Han and Hui old female patients with primary osteoporosis in Ningxia

作　　者：马宗军 闫军法 张汉霖 刘云宏 丁小力 马荣 于辛酉 戈朝晖 MA Zongjun;YAN Junfa;ZHANG Hanlin;LIU Yunhong;DING Xiaoli;MA Rong;YU Xinyou;GE Zhaohui(Department of Orthopedics,Cardio-Cerebrovascular Disease Hospital of General Hospital of Ningxia Medical University,Yinchuan 750002,Chin)

机构地区：[1]宁夏医科大学总医院心脑血管病医院骨科,宁夏银川750002

出　　处：《宁夏医学杂志》2018年第7期580-582,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ15184)

摘　　要：目的探讨宁夏汉族与回族老年女性原发性骨质疏松症降钙素受体(CTR)基因1377位点C/T多态性分布情况。方法收集女性原发性骨质疏松症患者200例,其中汉族100例,回族100例,采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)对所有患者的CTR基因进行多态性分析,探讨宁夏汉族与回族CTR基因多态性是否存在差异。结果 200例患者的CTR受体基因型频率分布均符合Hardy-Weinberg定律,汉族女性骨质疏松症患者组CTR基因型CC型占83.00%,CT型占17.00%;回族女性骨质疏松症患者组CC型占68.10%,CT型占22.90%,TT型占9.00%,2组比较差异有统计学意义(P<0.05);汉族女性骨质疏松症组C等位基因占91.5%,T等位基因占8.5%;回族女性骨质疏松症组C等位基因占79.55%,T等位基因占20.45%,2组比较差异有统计学意义(P<0.05)。结论宁夏汉族与回族女性原发性骨质疏松症患者CTR基因多态性存在民族差异。Objective To investigate the distribution of 1377 C/T polymorphism of calcitonin receptor（ CTR） gene between Han and Hui nationality population in Ningxia. Methods A total of 200 male outpatients were recruited,and divided into Han nationality osteoporosis group（ n = 100） and Han nationality osteoporosis group（ n = 100） according to the diagnostic criteria of osteoporosis. The genotype of CTR gene was examined by polymerase chain reaction-restriction fragment length polymorphism（ PCR-RELP） to identify the difference. Results The distribution of 1377-bp C/T single nucleotide polymorphism in calcitonin receptor gene was followed with the Hardy-Weinberg law. Genotypes CC,CT of CTR gene of Han female osteoporosis group were 83. 00%,17. 00%,and the genotypes in Hui female osteoporosis group were 68. 10%,22. 90% and 9. 00% respectively,and there were significant differences in the distribution of genotypes between these two groups（ P〈0. 05）. Allele Gene C and T of Han female osteoporosis group were 91. 5% and 8. 5%. Allele Gene C and T of Hui female osteoporosis group were 79. 55%,20. 45%. There was significant difference in the distribution of genotypes between these two groups（ P〈0. 05）. Conclusion There is a race difference in 1377 C/T single nucleotide polymorphism of calcitonin in female receptor gene between Han and Hui female patients.

关 键 词：降钙素基因受体多态性 汉族 回族 骨质疏松症 

分 类 号：R259[医药卫生—中西医结合]